Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad 500 607, India.
Bioresour Technol. 2010 Jul;101(14):5708-11. doi: 10.1016/j.biortech.2010.01.145. Epub 2010 Mar 1.
Efficient biohydrogen production from lignocellulosic hydrolysate assumes considerable practical and academic importance. The impact of glucose to xylose ratio, medium pH, inoculum size and age on biohydrogen production indicated that glucose to xylose ratio is the critical parameter for effective H(2) production compared to either pure glucose or xylose as carbon source. Inoculum size and its age contributed more than 70% to overall H(2) production and revealed significance at individual as well as interactive level. Maximum interaction of 39% and 32% was noticed with inoculum size and its age vs. glucose to xylose ratio (2:3), respectively. The H(2) production yield enhanced from 140 to 357 ml/g substrate upon statistical optimization revealing >240% improvement.
从木质纤维素水解物中高效生产生物氢气具有重要的实际意义和学术价值。葡萄糖与木糖比例、培养基 pH 值、接种物大小和年龄对生物氢气生产的影响表明，与纯葡萄糖或木糖作为碳源相比，葡萄糖与木糖的比例是有效生产 H2的关键参数。接种物大小及其年龄对总氢气产量的贡献超过 70%，并且在个体和相互作用水平上都具有重要意义。在与葡萄糖与木糖比例（2:3）的相互作用中，接种物大小和年龄分别观察到最大的相互作用为 39%和 32%。通过统计优化，氢气的产率从 140ml/g 底物提高到 357ml/g 底物，提高了>240%。
