Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, India.
Therdose PharmaPvt Limited, Hyderabad, India.
Indian J Microbiol. 2014 Sep;54(3):329-36. doi: 10.1007/s12088-014-0452-1. Epub 2014 Feb 22.
Production of protein encapsulated silver nanoparticles (AgNPs) assisted by marine actinomycetes strain has been investigated. The selective isolate was identified as Streptomyces parvulus SSNP11 based on chemotaxonomic and 16S rRNA analysis. Maximum AgNPs production was observed within 24 h incubation time. The produced AgNPs are spherical in shape with monodispersive and crystalline in nature. The particle size distribution ranges from 1.66 to 11.68 nm with a mean size of 2.1 nm. The biosynthesized AgNPs revealed stretching vibrations of primary and secondary amines along with C-H and C-N, suggesting that metabolically produced proteins are involved in size regulation of reduced AgNPs. These particles possess an average negative zeta potential value of 81.5 mV with an electrophoretic mobility of 0.000628 cm(2)/Vs. The biosynthesized nanoparticles revealed antimicrobial property against gram negative as well as gram positive bacterial strains.
利用海洋放线菌菌株辅助生产蛋白包裹的银纳米粒子（AgNPs）已经过研究。根据化学分类和 16S rRNA 分析，选择性分离株被鉴定为短小链霉菌 SSNP11。在 24 小时孵育时间内观察到最大的 AgNPs 产量。所产生的 AgNPs 呈球形，具有单分散性和结晶性。粒径分布范围为 1.66 至 11.68nm，平均粒径为 2.1nm。生物合成的 AgNPs 显示出伯胺和仲胺的伸缩振动以及 C-H 和 C-N，表明代谢产生的蛋白质参与了还原 AgNPs 的尺寸调节。这些颗粒具有平均负 zeta 电位值为 81.5mV，电泳迁移率为 0.000628cm(2)/Vs。生物合成的纳米粒子显示出对革兰氏阴性和革兰氏阳性细菌菌株的抗菌性能。
