海洋放线菌 SSNP11 产生并表征蛋白包被的银纳米粒子。
Production and Characterization of Protein Encapsulated Silver Nanoparticles by Marine Isolate Streptomyces parvulus SSNP11.
机构信息
Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad, India.
Therdose PharmaPvt Limited, Hyderabad, India.
出版信息
Indian J Microbiol. 2014 Sep;54(3):329-36. doi: 10.1007/s12088-014-0452-1. Epub 2014 Feb 22.
Abstract
Production of protein encapsulated silver nanoparticles (AgNPs) assisted by marine actinomycetes strain has been investigated. The selective isolate was identified as Streptomyces parvulus SSNP11 based on chemotaxonomic and 16S rRNA analysis. Maximum AgNPs production was observed within 24 h incubation time. The produced AgNPs are spherical in shape with monodispersive and crystalline in nature. The particle size distribution ranges from 1.66 to 11.68 nm with a mean size of 2.1 nm. The biosynthesized AgNPs revealed stretching vibrations of primary and secondary amines along with C-H and C-N, suggesting that metabolically produced proteins are involved in size regulation of reduced AgNPs. These particles possess an average negative zeta potential value of 81.5 mV with an electrophoretic mobility of 0.000628 cm(2)/Vs. The biosynthesized nanoparticles revealed antimicrobial property against gram negative as well as gram positive bacterial strains.
摘要
利用海洋放线菌菌株辅助生产蛋白包裹的银纳米粒子（AgNPs）已经过研究。根据化学分类和 16S rRNA 分析，选择性分离株被鉴定为短小链霉菌 SSNP11。在 24 小时孵育时间内观察到最大的 AgNPs 产量。所产生的 AgNPs 呈球形，具有单分散性和结晶性。粒径分布范围为 1.66 至 11.68nm，平均粒径为 2.1nm。生物合成的 AgNPs 显示出伯胺和仲胺的伸缩振动以及 C-H 和 C-N，表明代谢产生的蛋白质参与了还原 AgNPs 的尺寸调节。这些颗粒具有平均负 zeta 电位值为 81.5mV，电泳迁移率为 0.000628cm(2)/Vs。生物合成的纳米粒子显示出对革兰氏阴性和革兰氏阳性细菌菌株的抗菌性能。
相似文献
1
Production and Characterization of Protein Encapsulated Silver Nanoparticles by Marine Isolate Streptomyces parvulus SSNP11.海洋放线菌 SSNP11 产生并表征蛋白包被的银纳米粒子。
Indian J Microbiol. 2014 Sep;54(3):329-36. doi: 10.1007/s12088-014-0452-1. Epub 2014 Feb 22.
2
Characterization of silver nanoparticles synthesized by using marine isolate Streptomyces albidoflavus.利用海洋分离菌白黄链霉菌合成银纳米粒子的特性研究。
J Microbiol Biotechnol. 2012 May;22(5):614-21. doi: 10.4014/jmb.1107.07013.
3
Silver nanoparticles biosynthesized from secondary metabolite producing marine actinobacteria and evaluation of their biomedical potential.从产次级代谢产物的海洋放线菌生物合成的银纳米粒子及其生物医学潜力评价。
Antonie Van Leeuwenhoek. 2021 Oct;114(10):1497-1516. doi: 10.1007/s10482-021-01616-5. Epub 2021 Jul 29.
4
Antimicrobial, Antioxidant and Larvicidal Activities of Spherical Silver Nanoparticles Synthesized by Endophytic Streptomyces spp.内生链霉菌属合成的球形银纳米粒子的抗菌、抗氧化和杀幼虫活性
Biol Trace Elem Res. 2020 Jun;195(2):707-724. doi: 10.1007/s12011-019-01883-4. Epub 2019 Sep 5.
5
Biosynthesis of silver nanoparticles using actinobacterium Streptomyces albogriseolus and its antibacterial activity.利用 actinobacterium Streptomyces albogriseolus 合成银纳米粒子及其抗菌活性。
Biotechnol Appl Biochem. 2012 Nov-Dec;59(6):503-7. doi: 10.1002/bab.1054.
6
Fabrication of biogenic antimicrobial silver nanoparticles by Streptomyces aegyptia NEAE 102 as eco-friendly nanofactory.由埃及链霉菌 NEAE 102 生物合成抗菌银纳米粒子作为环保型纳米工厂。
J Microbiol Biotechnol. 2014 Apr;24(4):453-64. doi: 10.4014/jmb.1310.10095.
7
Anti-ESBL activity of silver nanoparticles biosynthesized using soil Streptomyces species.土壤链霉菌合成的银纳米粒子的抗 ESBL 活性。
Bioprocess Biosyst Eng. 2014 Jun;37(6):999-1006. doi: 10.1007/s00449-013-1070-8.
8
Studies on the impact of biosynthesized silver nanoparticles (AgNPs) in relation to malaria and filariasis vector control against Anopheles stephensi Liston and Culex quinquefasciatus Say (Diptera: Culicidae).关于生物合成银纳米粒子（AgNPs）对疟疾和丝虫病病媒控制的影响的研究，针对斯蒂芬斯按蚊和致倦库蚊（双翅目：蚊科）。
Parasitol Res. 2013 Feb;112(2):487-99. doi: 10.1007/s00436-012-3158-5. Epub 2012 Oct 12.
9
Biofabrication and spectral characterization of silver nanoparticles and their cytotoxic studies on human CD34 +ve stem cells.银纳米颗粒的生物制造、光谱表征及其对人CD34 +阳性干细胞的细胞毒性研究
3 Biotech. 2016 Dec;6(2):216. doi: 10.1007/s13205-016-0532-5. Epub 2016 Oct 6.
10
Antimicrobial Potential of Silver Nanoparticles Synthesized Using Medicinal Herb Coptidis rhizome.药用黄连根茎合成的银纳米粒子的抗菌潜力。
Molecules. 2018 Sep 5;23(9):2269. doi: 10.3390/molecules23092269.
引用本文的文献
1
Antimicrobial and antiproliferative activity of biosynthesized manganese nanocomposite with amide derivative originated by endophytic Aspergillus terreus.由内生土曲霉产生的具有酰胺衍生物的生物合成锰纳米复合材料的抗菌和抗增殖活性。
Microb Cell Fact. 2025 Feb 4;24(1):37. doi: 10.1186/s12934-025-02651-x.
2
Antibacterial Therapy by Ag Ions Complexed with Titan Yellow/Congo Red and Albumin during Anticancer Therapy of Urinary Bladder Cancer.载银钛黄/刚果红-白蛋白复合物用于膀胱癌抗癌治疗中的抗菌治疗。
Int J Mol Sci. 2021 Dec 21;23(1):26. doi: 10.3390/ijms23010026.
3
Green Synthesized Silver Nanoparticles: Antibacterial and Anticancer Activities, Biocompatibility, and Analyses of Surface-Attached Proteins.绿色合成银纳米颗粒：抗菌和抗癌活性、生物相容性以及表面附着蛋白分析
Front Microbiol. 2021 Apr 22;12:632505. doi: 10.3389/fmicb.2021.632505. eCollection 2021.
4
Genome guided investigation of antibiotics producing actinomycetales strain isolated from a Macau mangrove ecosystem.从澳门红树林生态系统中分离的产抗生素放线菌菌株的基因组指导研究。
Sci Rep. 2018 Sep 24;8(1):14271. doi: 10.1038/s41598-018-32076-z.
5
Biosynthesis of Ag, Se, and ZnO nanoparticles with antimicrobial activities against resistant pathogens using waste isolate .利用废弃分离物合成具有抗耐药病原体抗菌活性的银、硒和氧化锌纳米粒子。
IET Nanobiotechnol. 2018 Sep;12(6):741-747. doi: 10.1049/iet-nbt.2017.0213.
6
Synthesis, characterisation and bactericidal effect of ZnO nanoparticles via chemical and bio-assisted ( in vitro plantlets and callus extract) methods: a comparative study.通过化学和生物辅助（体外幼苗和愈伤组织提取物）方法合成、表征及研究ZnO纳米颗粒的杀菌效果：一项对比研究。
IET Nanobiotechnol. 2018 Aug;12(5):604-608. doi: 10.1049/iet-nbt.2017.0067.
7
Biosynthesis and antibacterial activity of ZnO nanoparticles using Trifolium pratense flower extract.利用红车轴草花提取物合成氧化锌纳米颗粒及其抗菌活性
Saudi J Biol Sci. 2016 Jul;23(4):517-23. doi: 10.1016/j.sjbs.2015.05.016. Epub 2015 May 31.
8
Antimicrobial Activities of Silver Nanoparticles Synthesized by Using Water Extract of Arnicae anthodium.采用艾纳香头状花序水提物合成的纳米银的抗菌活性
Indian J Microbiol. 2015 Jun;55(2):168-74. doi: 10.1007/s12088-015-0516-x. Epub 2015 Feb 7.
本文引用的文献
1
Characterization of silver nanoparticles synthesized by using marine isolate Streptomyces albidoflavus.利用海洋分离菌白黄链霉菌合成银纳米粒子的特性研究。
J Microbiol Biotechnol. 2012 May;22(5):614-21. doi: 10.4014/jmb.1107.07013.
2
Biohydrogen production process optimization using anaerobic mixed consortia: a prelude study for use of agro-industrial material hydrolysate as substrate.利用厌氧混合菌群优化生物制氢工艺：以农业工业材料水解物为底物的前期研究。
Bioresour Technol. 2010 Jul;101(14):5708-11. doi: 10.1016/j.biortech.2010.01.145. Epub 2010 Mar 1.
3
Biological synthesis of metal nanoparticles by microbes.微生物生物合成金属纳米粒子。
Adv Colloid Interface Sci. 2010 Apr 22;156(1-2):1-13. doi: 10.1016/j.cis.2010.02.001. Epub 2010 Feb 10.
4
Biomimetic synthesis and characterisation of protein capped silver nanoparticles.蛋白质包覆银纳米颗粒的仿生合成与表征
Bioresour Technol. 2009 Jan;100(1):501-4. doi: 10.1016/j.biortech.2008.05.048. Epub 2008 Jul 14.
5
Biosynthesis of silver nanocrystals by Bacillus licheniformis.地衣芽孢杆菌合成银纳米晶体
Colloids Surf B Biointerfaces. 2008 Aug 1;65(1):150-3. doi: 10.1016/j.colsurfb.2008.02.018. Epub 2008 Mar 4.
6
Biosorption and bioreduction of trivalent aurum by photosynthetic bacteria Rhodobacter capsulatus.光合细菌荚膜红细菌对三价金的生物吸附与生物还原作用
Curr Microbiol. 2007 Nov;55(5):402-8. doi: 10.1007/s00284-007-9007-6. Epub 2007 Aug 23.
7
Sulfite reductase-mediated synthesis of gold nanoparticles capped with phytochelatin.亚硫酸盐还原酶介导的植物螯合肽包覆金纳米颗粒的合成。
Biotechnol Appl Biochem. 2007 Aug;47(Pt 4):191-5. doi: 10.1042/BA20060205.
8
Extracellular biosynthesis of bimetallic Au-Ag alloy nanoparticles.双金属金-银合金纳米颗粒的细胞外生物合成。
Small. 2005 May;1(5):517-20. doi: 10.1002/smll.200400053.
9
Studies on the cell-wall composition and taxonomy of Actinomycetales and related groups.放线菌目及相关类群的细胞壁组成与分类研究。
J Gen Microbiol. 1958 Feb;18(1):173-89. doi: 10.1099/00221287-18-1-173.
10
Reduced transition metal colloids: a novel family of reusable catalysts?还原态过渡金属胶体：一类新型的可重复使用催化剂？
Chem Rev. 2002 Oct;102(10):3757-78. doi: 10.1021/cr010350j.
Zotero 插件