Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran 14174, Iran.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Apr 1;878(11-12):845-52. doi: 10.1016/j.jchromb.2010.01.040. Epub 2010 Jan 29.
Providing "real blank sample" is a problem in determination of endogenous steroids in complex matrices. A new quantification strategy is proposed in the present study, which is based on using isotope-labeled steroids instead of natural steroids for constructing calibration line. This approach is called surrogate analyte and it is shown that its accuracy is better than some of the previously described methods at low concentrations and comparable to standard addition method at medium and high concentration levels. The method was fully validated to satisfy the ICH criteria and it was applied for determination of endogenous steroids in several urine samples.
在复杂基质中测定内源性甾体时，提供“真实空白样本”是一个问题。本研究提出了一种新的定量策略，该策略基于使用同位素标记的甾体代替天然甾体来构建校准曲线。这种方法称为替代分析物，结果表明，与低浓度下的一些先前描述的方法相比，该方法的准确性更好，与中高浓度水平下的标准添加法相当。该方法经过全面验证，符合 ICH 标准，并应用于几种尿液样本中内源性甾体的测定。
