Department of Clinical Analysis, State University of Maringá, Maringá, Paraná, Brazil.
J Altern Complement Med. 2010 Mar;16(3):285-90. doi: 10.1089/acm.2009.0281.
The objective of this study was to evaluate the in vitro antifungal activity of propolis extract against yeasts Candida albicans and Candida non-albicans isolated from vaginal exudates, in comparison with nystatin.
Ninety-seven (97) vaginal yeasts strains were evaluated. These strains were obtained from different clinical conditions, isolated and stored at the Sector of Medical Mycology of the State University of Maringá (Paraná, Brazil). The assays of susceptibility to nystatin and propolis extracts (PE) were conducted through microdilution in broth (National Committee for Clinical Laboratory Standards-NCCLS, M-27A Document of 1997).
All the yeasts tested were inhibited by low concentrations of PE (maximum of 393.19 mug/mL of the total flavonoid content), including an isolate resistant to nystatin, regardless of the clinical conditions of the women and the species of yeast isolated.
The PE showed an outstanding performance against the tested vaginal yeast strains, and could be included among the novel therapeutic options for the treatment of vulvovaginal candidiasis.
本研究旨在评估蜂胶提取物对阴道分泌物中分离出的白色念珠菌和非白色念珠菌的体外抗真菌活性，并与制霉菌素进行比较。
评估了 97 株阴道酵母菌株。这些菌株是从不同的临床条件下分离出来的，并储存在马兰戈州立大学医学真菌学部门（巴西巴拉那州）。通过肉汤微量稀释法（国家临床实验室标准委员会，1997 年 M-27A 文件）进行对制霉菌素和蜂胶提取物（PE）的药敏试验。
所有测试的酵母菌均被低浓度的 PE（总黄酮含量最高 393.19 微克/毫升）抑制，包括对制霉菌素耐药的分离株，无论女性的临床状况和分离的酵母菌种类如何。
PE 对测试的阴道酵母菌株表现出出色的性能，可作为治疗外阴阴道念珠菌病的新治疗选择之一。
