亚热带水域中大肠杆菌的计数方法。
Methods for enumerating Escherichia coli in subtropical waters.
作者信息
Cheung W H, Ha D K, Yeung K Y, Hung R P
机构信息
Environmental Protection Department, Southorn Centre, Wanchai, Hong Kong.
出版信息
Epidemiol Infect. 1991 Apr;106(2):345-54. doi: 10.1017/s0950268800048494.
The standard membrane filtration method of the UK has been modified in order to improve its specificity for enumerating Escherichia coli in the subtropical waters of Hong Kong. This involves incorporating into the membrane lauryl sulphate (mLS) method either an in situ urease test (the mLS-UA method), or an in situ beta-glucuronidase test (the mLS-GUD method). The false-positive errors of the mLS-UA and mLS-GUD methods are low, ranging from 3-5%. A comparison between the membrane filtration (mLS-UA) method and the multiple tube technique in testing E. coli in subtropical beach-waters has demonstrated that the former can give much more precise counts, and is the method of choice for such a purpose. The mLS-GUD method, for which automated counting of E. coli colonies is possible, is a good alternative to mLS-UA in routine enumeration of this bacterial indicator in environmental waters.
英国的标准膜过滤法已作修改，以提高其在香港亚热带水域中对大肠杆菌计数的特异性。这涉及在膜十二烷基硫酸盐（mLS）法中加入原位尿素酶试验（mLS-UA法）或原位β-葡萄糖醛酸酶试验（mLS-GUD法）。mLS-UA法和mLS-GUD法的假阳性误差较低，在3%-5%之间。膜过滤（mLS-UA）法与多管技术在亚热带海滩水域检测大肠杆菌的比较表明，前者能给出更精确的计数，是用于此目的的首选方法。mLS-GUD法可对大肠杆菌菌落进行自动计数，是环境水体中该细菌指标常规计数时mLS-UA法的良好替代方法。
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