氢交换质谱法：它是什么，能告诉我们什么？
Hydrogen exchange mass spectrometry: what is it and what can it tell us?
机构信息
The Department of Chemistry & Chemical Biology and The Barnett Institute of Chemical & Biological Analysis, Northeastern University, Boston, MA 02115, USA.
出版信息
Anal Bioanal Chem. 2010 Jun;397(3):967-72. doi: 10.1007/s00216-010-3556-4. Epub 2010 Mar 1.
Abstract
Proteins are undoubtedly some of the most essential molecules of life. While much is known about many proteins, some aspects still remain mysterious. One particularly important aspect of understanding proteins is determining how structure helps dictate function. Continued development and implementation of biophysical techniques that provide information about protein conformation and dynamics is essential. In this review, we discuss hydrogen exchange mass spectrometry and how this method can be used to learn about protein conformation and dynamics. The basic concepts of the method are described, the workflow illustrated, and a few examples of its application are provided.
摘要
蛋白质无疑是生命中最重要的分子之一。虽然人们对许多蛋白质有了很多了解，但仍有一些方面还很神秘。了解蛋白质的一个特别重要的方面是确定结构如何帮助决定功能。继续开发和实施提供有关蛋白质构象和动力学信息的生物物理技术至关重要。在这篇综述中，我们讨论了氢交换质谱法，以及如何使用这种方法来了解蛋白质的构象和动力学。描述了该方法的基本概念，说明了工作流程，并提供了一些应用实例。
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