Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
Curr Microbiol. 2010 Oct;61(4):274-9. doi: 10.1007/s00284-010-9607-4. Epub 2010 Feb 27.
The antifungal activity of silicon (Si) on Penicillium digitatum, and the possible action mode involved were investigated. Spore germination, germ tube elongation, and mycelial growth of P. digitatum were strongly inhibited by Si in the form of sodium silicate. Using propidium iodide (PI) stain combined with fluorescent microscopy, it was found that the plasma membrane of Si-treated P. digitatum spores was obviously damaged, and the leakage of protein and sugar was significantly higher in Si-treated mycelia than that of control. These findings suggest that the damage on plasma membrane of P. digitatum played a crucial role in the antifungal effect of Si. Moreover, Si was effective in controlling green mold caused by P. digitatum in citrus fruit. These results have a beneficial impact on the application of Si in the control of postharvest diseases.
研究了硅（Si）对柑橘绿霉病病原菌指状青霉（Penicillium digitatum）的抑菌活性及其可能的作用模式。以硅酸钠形式存在的硅强烈抑制指状青霉的孢子萌发、芽管伸长和菌丝生长。使用碘化丙啶（PI）染色结合荧光显微镜观察发现，硅处理的指状青霉孢子的质膜明显受损，且硅处理的菌丝中蛋白质和糖的渗漏明显高于对照。这些结果表明，指状青霉质膜的损伤在硅的抑菌作用中起关键作用。此外，硅在控制柑橘果实中青霉病的发生方面是有效的。这些结果对硅在采后病害防治中的应用具有有益的影响。
