Geiger O, Weissborn A C, Kennedy E P
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115.
J Bacteriol. 1991 May;173(9):3021-4. doi: 10.1128/jb.173.9.3021-3024.1991.
Cyclic beta-1,2-glucans produced by Agrobacterium and Rhizobium species play an important role in the interaction of these bacteria with plant hosts. In this study, we show that (i) the neutral cyclic glucans are the biosynthetic precursors of anionic cyclic glucans; (ii) the conversion of neutral to anionic glucans is much more rapid and more extensive in exponentially growing cultures than in cultures in the stationary phase, although the latter synthesize large amounts of glucan; and (iii) the excretion of glucan, as well as the total amount synthesized, is strongly influenced by the medium.
农杆菌属和根瘤菌属产生的环状β-1,2-葡聚糖在这些细菌与植物宿主的相互作用中发挥着重要作用。在本研究中，我们发现：（i）中性环状葡聚糖是阴离子环状葡聚糖的生物合成前体；（ii）在指数生长期培养物中，中性葡聚糖向阴离子葡聚糖的转化比稳定期培养物中更快、更广泛，尽管后者合成大量葡聚糖；（iii）葡聚糖的分泌以及合成总量受培养基的强烈影响。
