Department of Plant Biology and Bell Museum of Natural History, University of Minnesota, 1445 Gortner Ave, St. Paul, MN 55108, USA;
Persoonia. 2009 Dec;23:35-40. doi: 10.3767/003158509X470602. Epub 2009 Aug 4.
Developing powerful phylogenetic markers is a key concern in fungal phylogenetics. Here we report degenerate primers that amplify the single-copy genes Mcm7 (MS456) and Tsr1 (MS277) across a wide range of Pezizomycotina (Ascomycota). Phylogenetic analyses of 59 taxa belonging to the Eurotiomycetes, Lecanoromycetes, Leotiomycetes, Lichinomycetes and Sordariomycetes, indicate the utility of these loci for fungal phylogenetics at taxonomic levels ranging from genus to class. We also tested the new primers in silico using sequences of Saccharomycotina, Taphrinomycotina and Basidiomycota to predict their potential of amplifying widely across the Fungi. The analyses suggest that the new primers will need no, or only minor sequence modifications to amplify Saccharomycotina, Taphrinomycotina and Basidiomycota.
开发强大的系统发育标记是真菌系统发生学的一个关键关注点。在这里，我们报告了简并引物，可在广泛的 Pezizomycotina（子囊菌门）中扩增单拷贝基因 Mcm7（MS456）和 Tsr1（MS277）。对属于 Eurotiomycetes、Lecanoromycetes、Leotiomycetes、Lichinomycetes 和 Sordariomycetes 的 59 个分类群的系统发育分析表明，这些基因座在从属到纲的分类水平上对真菌系统发生学具有实用性。我们还使用 Saccharomycotina、Taphrinomycotina 和 Basidiomycota 的序列进行了计算机测试，以预测这些新引物在真菌中广泛扩增的潜力。分析表明，新引物将无需或仅需进行较小的序列修改即可扩增 Saccharomycotina、Taphrinomycotina 和 Basidiomycota。
