North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, Manchester M13 3PT, UK.
In Vitro Cell Dev Biol Anim. 2010 Apr;46(3-4):386-94. doi: 10.1007/s11626-010-9291-5. Epub 2010 Feb 23.
We report here the derivation of two new human embryonic stem cell lines, Man-1 and Man-2, and their full characterization as novel pluripotent stem cell lines. Man-1 was derived from an embryo surplus to requirement from routine IVF, while Man-2 was obtained from an oocyte classified as failed to fertilise and subsequently chemically activated. We report the characterisation of pluripotency and the differentiation potential of these lines. Work is in progress to establish novel methods of stem cell derivation and culture, which will avoid the use of xenobiotics and be relevant to clinical production of human embryonic stem cell lines. Both newly derived human embryonic stem cell lines will be available for the research community from the UK Stem Cell Bank (http://www.ukstemcellbank.org.uk).
我们在此报告两个新的人类胚胎干细胞系 Man-1 和 Man-2 的衍生，并对它们作为新型多能干细胞系的特征进行全面描述。Man-1 是从常规体外受精（IVF）多余的胚胎中衍生而来的，而 Man-2 则是从未受精的卵母细胞中获得的，并随后进行化学激活。我们报告了这些细胞系的多能性和分化潜能的特征。目前正在开展工作以建立新的干细胞衍生和培养方法，这些方法将避免使用异源物，并与人类胚胎干细胞系的临床生产相关。这两个新衍生的人类胚胎干细胞系将可从英国干细胞银行（http://www.ukstemcellbank.org.uk）获得，供研究界使用。
