Bauru Dental School, University of São Paulo, Department of Biological Sciences, Al. Octávio Pinheiro Brisolla, 9-75, Bauru-SP 17012-901, Brazil.
J Dent Res. 2010 May;89(5):468-72. doi: 10.1177/0022034510363248. Epub 2010 Mar 3.
Matrix metalloproteinase (MMP) inhibition has been shown to reduce dentin caries progression, but its role in dental erosion has not yet been assessed. This study tested the hypothesis that gels containing MMP inhibitors (epigallocatechin gallate-EGCG and chlorhexidine) can prevent dental erosion. Volunteers (n = 10) wore palatal devices containing bovine dentin blocks (n = 10/group) treated for 1 min with EGCG at 10 (EGCG10) or 400 microM (EGCG400), chlorhexidine at 0.012%, F at 1.23% (NaF), and no vehicle (placebo). Erosion was performed with Coca-Cola (5 min) 4X/day during 5 days. The wear, assessed by profilometry (mean +/- SD, microm), was significantly reduced by the gels containing MMP inhibitors (0.05 +/- 0.02(a), 0.04 +/- 0.02(a), and 0.05 +/- 0.02(a) for EGCG10, EGCG400, and chlorhexidine, respectively) when compared with NaF (0.79 +/- 0.35(b)) and placebo gels (1.77 +/- 0.35(b)) (Friedman and Dunn's tests, p < 0.01). The use of gels delivering MMP inhibitors was shown to prevent erosion and opens a new perspective for protection against dental erosion.
基质金属蛋白酶 (MMP) 抑制已被证明可减少牙本质龋的进展，但它在牙齿酸蚀中的作用尚未得到评估。本研究检验了这样一个假设，即含有 MMP 抑制剂（表没食子儿茶素没食子酸酯-EGCG 和洗必泰）的凝胶可以预防牙齿酸蚀。志愿者（n = 10）佩戴含有牛牙本质块的腭部装置（n = 10/组），用 EGCG 处理 1 分钟，浓度分别为 10（EGCG10）或 400 microM（EGCG400）、0.012%洗必泰、1.23%F（NaF）和无载体（安慰剂）。用可口可乐（5 分钟）每天 4 次进行酸蚀，持续 5 天。用轮廓仪评估的磨损（平均值 +/- SD，微米），用含有 MMP 抑制剂的凝胶显著减少（分别为 0.05 +/- 0.02(a)、0.04 +/- 0.02(a)和 0.05 +/- 0.02(a)），与 NaF（0.79 +/- 0.35(b))和安慰剂凝胶（1.77 +/- 0.35(b))相比（Friedman 和 Dunn 检验，p < 0.01）。使用递送 MMP 抑制剂的凝胶被证明可以预防酸蚀，为预防牙齿酸蚀开辟了新的前景。
