de Luis D A, González Sagrado M, Aller R, Izaola O, Conde R, Romero E
Institute of Endocrinology and Nutrition, Medicine School, University of Valladolid, Simancas, Valladolid, Spain.
Nutr Hosp. 2010 Jan-Feb;25(1):34-8.
A silent intragenic biallelic polymorphism (1359 G/A) (rs1049353) of the CB1 gene resulting in the substitution of the G to A at nucleotide position 1359 in codon 435 (Thr), was reported as a common polymorphism in Caucasian populations.
The aim of our study was to investigate the influence of the missense polymorphism (G1359A) of CB1 receptor gene on obesity anthropometric parameters, cardiovascular risk factors and adipocytokines in patients with obesity and diabetes mellitus type 2.
A population of 60 naïve diabetic patients was analyzed. An indirect calorimetry, tetrapolar electrical bioimpedance, blood pressure, a serial assessment of nutritional intake with 3 days written food records and biochemical analysis (lipid profile, adipocytokines, insulin, CRP and lipoprotein-a) were performed. The statistical analysis was performed for the combined G1359A and A1359A as a group and wild type G1359G as second group, with a dominant model.
The mean age was 57.44 +/- 11.7 years and the mean BMI 37.84 +/- 6.4, with 14 males and 46 females. Thirty-five patients (58.3%) had genotype G1359G (wild type group) and 25 (42.7%) patients G1359A (mutant type group). Age was similar in both groups (wild type: 56.3 +/- 11.8 years vs mutant group: 58.7 +/- 10 years:ns). Sex distribution was similar in both groups (wild vs mutant type groups), males (22.9% vs 24%) and females (77.1% vs 76%). No differences were detected between groups in anthropometric parameters, cardiovascular risk factors, dietary intake and adipocytokines levels.
The finding of this study is the lack of association of G1359A polymorphism of CB receptor 1 gene with obesity, cardiovascular risk factors and adipocytokines.
据报道，CB1基因的一种沉默基因内双等位基因多态性（1359G/A）（rs1049353）导致密码子435（苏氨酸）中第1359位核苷酸的G被A取代，是白种人群中的一种常见多态性。
我们研究的目的是调查CB1受体基因错义多态性（G1359A）对肥胖症和2型糖尿病患者的肥胖人体测量参数、心血管危险因素和脂肪细胞因子的影响。
分析了60例初发糖尿病患者群体。进行了间接测热法、四极生物电阻抗法、血压测量、通过3天书面饮食记录对营养摄入进行连续评估以及生化分析（血脂谱、脂肪细胞因子、胰岛素、CRP和脂蛋白-a）。采用显性模型，将G1359A和A1359A合并为一组，野生型G1359G作为第二组进行统计分析。
平均年龄为57.44±11.7岁，平均BMI为37.84±6.4，其中男性14例，女性46例。35例患者（58.3%）具有基因型G1359G（野生型组），25例患者（42.7%）具有G1359A（突变型组）。两组年龄相似（野生型：56.3±11.8岁，突变型组：58.7±10岁：无显著性差异）。两组性别分布相似（野生型与突变型组），男性（22.9%对24%）和女性（77.1%对76%）。两组在人体测量参数、心血管危险因素、饮食摄入和脂肪细胞因子水平方面未检测到差异。
本研究的发现是CB1受体1基因的G1359A多态性与肥胖、心血管危险因素和脂肪细胞因子之间缺乏关联。
