State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, No 79 Qing Chun Road, Hangzhou, Zhejiang Province, 310003 People's Republic of China.
Eur J Clin Microbiol Infect Dis. 2010 May;29(5):613-6. doi: 10.1007/s10096-010-0893-3. Epub 2010 Mar 4.
The aim of this study was to perform the molecular characterization of methicillin-resistant Staphylococcus haemolyticus (MRSH) from clinical specimens of patients in a Chinese hospital. One hundred and thirty-three strains of S. haemolyticus collected from April 2002 to April 2003 were analyzed. Antimicrobial susceptibility to 15 antimicrobial agents was determined by the broth microdilution method. The resistant rates to penicillin G and oxacillin were higher than 90%. There were no isolates resistant to linezolid or vancomycin, and only 6.0% of the strains were resistant to teicoplanin. The positivity rate for mecA genes was 90.2% by polymerase chain reaction (PCR). Ninety MRSH (isolated from inpatients and mecA-gene-positive) were genotyped by pulsed-field gel electrophoresis (PFGE) after SmaI digestion. Twenty-five different PFGE patterns (A approximately Y) were found and a major clone (type A; n = 36) with five subtypes was identified. Clone A was detected during a 1-year period. Identical PFGE types were found in different wards and patients. The results of this study suggest the clonal spread of MRSH within our hospital. This emphasizes the need for control and prevention measures.
本研究旨在对中国医院临床标本中分离的耐甲氧西林溶血葡萄球菌（MRSH）进行分子特征分析。对 2002 年 4 月至 2003 年 4 月期间收集的 133 株溶血葡萄球菌进行分析。采用肉汤微量稀释法测定了 15 种抗菌药物的药敏性。青霉素 G 和苯唑西林的耐药率均高于 90%。未发现对利奈唑胺或万古霉素耐药的分离株，仅有 6.0%的菌株对替考拉宁耐药。聚合酶链反应（PCR）检测 mecA 基因的阳性率为 90.2%。对 90 株（住院患者且 mecA 基因阳性）MRSH 进行 SmaI 消化后的脉冲场凝胶电泳（PFGE）基因分型。发现了 25 种不同的 PFGE 模式（A 至 Y），并鉴定出一种主要克隆（A型；n=36），其中有 5 种亚型。A 型克隆在一年内被检测到。不同病房和患者中发现了相同的 PFGE 类型。本研究结果表明 MRSH 在我院内存在克隆传播。这强调了需要采取控制和预防措施。
