Graduate School of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya, Japan.
Inflamm Res. 2010 Aug;59(8):615-25. doi: 10.1007/s00011-010-0173-9. Epub 2010 Mar 5.
We investigated the effects of hydrolyzed rice bran (HRB), an arabinoxylan extracted from rice bran, on mast cell degranulation and cytokine production.
HRB was obtained by treating rice bran with an extract obtained from shiitake mushrooms. Bone marrow-derived mast cells (BMMCs) were prepared by culturing bone marrow cells from BALB/c mice in the presence of interleukin-3 and stem cell factor for 4 weeks. BMMCs were pretreated with HRB (0-3 mg/ml) for 30 min and were then antigen activated.
Pretreatment of BMMCs with HRB significantly inhibited antigen-induced degranulation and cytokine production (tumor necrosis factor-alpha and interleukin-4) in a dose-dependent manner. HRB also diminished membrane fusion between liposomes in which soluble N-ethyl maleimide-sensitive factor attachment protein receptors were reconstituted. Phosphorylation of RelA and mitogen-activated kinases after antigen stimulation was suppressed by pretreatment of BMMCs with HRB.
These findings suggest that HRB may have an anti-inflammatory effect by inhibiting mast cell degranulation and cytokine production.
研究从米糠中提取的阿拉伯木聚糖水解米糠（HRB）对肥大细胞脱颗粒和细胞因子产生的影响。
HRB 通过用香菇提取物处理米糠获得。通过在白细胞介素-3 和干细胞因子存在的情况下培养来自 BALB/c 小鼠的骨髓细胞 4 周来制备骨髓来源的肥大细胞（BMMC）。BMMC 用 HRB（0-3mg/ml）预处理 30min 后，再进行抗原激活。
HRB 预处理可显著抑制 BMMC 中抗原诱导的脱颗粒和细胞因子（肿瘤坏死因子-α和白细胞介素-4）的产生，呈剂量依赖性。HRB 还减少了其中重新构成可溶性 N-乙基马来酰亚胺敏感因子附着蛋白受体的脂质体之间的膜融合。HRB 预处理可抑制 BMMC 中抗原刺激后的 RelA 和丝裂原激活蛋白激酶的磷酸化。
这些发现表明，HRB 通过抑制肥大细胞脱颗粒和细胞因子产生可能具有抗炎作用。
