与噬菌体Mu和D108的ner基因高度同源的大肠杆菌基因nlp的克隆与测序。
Cloning and sequencing of an Escherichia coli gene, nlp, highly homologous to the ner genes of bacteriophages Mu and D108.
作者信息
Choi Y L, Nishida T, Kawamukai M, Utsumi R, Sakai H, Komano T
机构信息
Department of Agricultural Chemistry, Kyoto University, Japan.
出版信息
J Bacteriol. 1989 Sep;171(9):5222-5. doi: 10.1128/jb.171.9.5222-5225.1989.
Abstract
An nlp (Ner-like protein) gene was isolated from Escherichia coli. The nucleotide sequence of a 1,342-base-pair chromosomal DNA fragment containing the nlp gene was analyzed. It contained two open reading frames; one encoded 91 amino acid residues with an Mr of 10,361, and the other (ORFX) encoded 131 amino acid residues of the carboxyl-terminal region of a truncated polypeptide. The amino acid sequence deduced from the DNA sequence of nlp was highly homologous (62 to 63%) to the Ner proteins of bacteriophages Mu and D108. The amino-terminal region of Nlp deduced from the complete open reading frame contained a presumed DNA-binding region. The nlp gene was located at 69.3 min on the E. coli genetic map.
摘要
从大肠杆菌中分离出一个nlp（类Ner蛋白）基因。对包含nlp基因的1342个碱基对的染色体DNA片段的核苷酸序列进行了分析。它包含两个开放阅读框；一个编码91个氨基酸残基，Mr为10361，另一个（ORFX）编码截短多肽羧基末端区域的131个氨基酸残基。从nlp的DNA序列推导的氨基酸序列与噬菌体Mu和D108的Ner蛋白高度同源（62%至63%）。从完整开放阅读框推导的Nlp氨基末端区域包含一个推测的DNA结合区域。nlp基因位于大肠杆菌遗传图谱的69.3分钟处。
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