Department of Chemistry, Hong Kong Baptist University, Kowloon, Hong Kong SAR, China.
Chin Med. 2010 Jan 29;5:4. doi: 10.1186/1749-8546-5-4.
Antrodia camphorata (AC) is an important fungus native to Taiwanese forested regions. Scientific studies have demonstrated that extracts of AC possess a variety of pharmacological functions. This study aims to identify the full profile fingerprint of nucleosides and nucleobases in mycelial AC and to assess the quality of two commercial mycelial AC products.
High-performance liquid chromatography coupled with diode array detector and mass spectrometry was employed to identify the major components in mycelial AC. The chemical separation was carried out using a gradient program on a reverse phase Alltima C18 AQ analytical column (250 x 4.6 mm, 5 microm) with the mobile phase consisting of deionized water and methanol.
Ten nucleosides and nucleobases, two maleimide derivatives, and a sterol were identified as the major constituents in mycelial AC. These groups of chemical compounds constitute the first chromatographic fingerprint as an index for quality assessment of this medicinal fungus.
This study provides the first chromatographic fingerprint to assess the quality of mycelial AC.
樟芝（Antrodia camphorata，AC）是一种原产于台湾森林地区的重要真菌。科学研究表明，AC 的提取物具有多种药理作用。本研究旨在鉴定菌丝体 AC 中核苷和碱基的全谱指纹图谱，并评估两种商业菌丝体 AC 产品的质量。
采用高效液相色谱-二极管阵列检测器-质谱联用技术鉴定菌丝体 AC 的主要成分。采用梯度程序在 Alltima C18 AQ 分析柱（250×4.6mm，5μm）上进行化学分离，流动相为去离子水和甲醇。
鉴定出 10 种核苷和碱基、2 种马来酰亚胺衍生物和一种甾醇，作为菌丝体 AC 的主要成分。这些化合物群构成了第一个色谱指纹图谱，作为该药用真菌质量评估的指标。
本研究提供了首个色谱指纹图谱来评估菌丝体 AC 的质量。
