The important zoonoses in the protected areas of the Tatra National Park (TANAP).

The northern part of Slovakia constitutes an important tourist and recreational area of the country. Protected localities of the Tatra National Park (TANAP) are characterised by specific ecological conditions. The high numbers of animals inhabiting protected areas of the TANAP and their potential encounters with tourists contribute to a risk of transmission of important parasitic zoonoses. The aim of presently reported study was to perform a long-lasting and detailed survey on the occurrence of zoonotic agents (in particular Echinococcus multilocularis and Trichinella spp.) with particular emphasis on the interactions between the environment, the host, and the parasite as well as the adaptation mechanisms in individual ecosystems. Within the pilot part of the study, which started in 2007, in total 397 samples of wild predators representing 10 species belonging to families Canidae, Felidae, Mustelidae and Ursidae were investigated. Helminthological necropsy (modified sedimentation and counting technique) and artificial digestion method were used for the parasites detection. Multiplex PCR approach has been used for species identification of Trichinella isolates obtained from infected animals. E. multilocularis was found in 42.7% of foxes and 1 raccoon dog. Trichinella larvae were present in 16.7% of foxes, 37.9% of martens, 33.3% of polecats, 1 bear and 1 lynx. All animals were infected by T. britovi.