Geng Dongsheng, Zhang Shufeng, Lan Jianguo
Institute for Drug and Instrument Control of Xinjiang Military Command, Urumqi 830063, China.
Zhongguo Zhong Yao Za Zhi. 2009 Nov;34(22):2887-90.
To analysis chemical components of volatile oil from the seed of Nigella glandulifera (NG), comparing them with those from the seed of foreign N. sativa (NS) and N. damascene (ND), and to quantify thymoquinone in the volatile oil extracted by hydrodistillation (HD) from the seed of NG.
The volatile oil was extracted by supercritical CO2 extraction (SFE-CO2 ) and HD from the seed of NG and its chemical components was analysed by GC-MS, the relative percentage of components were determined by peak aera normalization method and compare with those of the seed of NS and ND. The content of the thymoquinone in the volatile oil was determined by one point external standard method.
In terms of the volatile compounds, p-cymene is the major component of NG and NS, their relative percentage contents are 33.75% and 61.48%, respectively. beta-Elemene is the major component of ND, its relative percentage content is 73.24%. The relative percentage contents of thymoquinone are 3.73% (HD), 3.80% and 0.08% in NG, NS and ND, respectively. Linoleic acid is a major component of volatile oil by SFE-CO2 in NG, but its content of p-cymene is lower. The absolute percentage content of thymoquinone is 1.58% by HD in volatile oil of NG.
There are comparatively large differences of volatile components in NG, NS and ND.
分析腺毛黑种草籽挥发油的化学成分，并与国外的黑种草籽和大马士革黑种草籽进行比较，同时对腺毛黑种草籽水蒸馏法提取挥发油中的百里醌进行定量分析。
采用超临界CO2萃取法（SFE-CO2）和水蒸馏法（HD）从腺毛黑种草籽中提取挥发油，用气相色谱-质谱联用（GC-MS）分析其化学成分，采用峰面积归一化法测定各成分的相对百分含量，并与黑种草籽和大马士革黑种草籽进行比较。采用单点外标法测定挥发油中百里醌的含量。
在挥发性成分方面，对伞花烃是腺毛黑种草籽和黑种草籽的主要成分，其相对百分含量分别为33.75%和61.48%。β-榄香烯是大马士革黑种草籽的主要成分，其相对百分含量为73.24%。百里醌在腺毛黑种草籽、黑种草籽和大马士革黑种草籽中的相对百分含量分别为3.73%（HD）、3.80%和0.08%。亚油酸是腺毛黑种草籽超临界CO2萃取挥发油的主要成分，但对伞花烃含量较低。腺毛黑种草籽挥发油水蒸馏法提取的百里醌绝对百分含量为1.58%。
腺毛黑种草籽、黑种草籽和大马士革黑种草籽的挥发性成分存在较大差异。
