肿瘤中血管内皮生长因子的荧光成像：用于混浊介质中嵌入的小鼠。
Fluorescence imaging of vascular endothelial growth factor in tumors for mice embedded in a turbid medium.
机构信息
University of Connecticut, Department of Electrical and Computer Engineering, Storrs, Connecticut 06269, USA.
出版信息
J Biomed Opt. 2010 Jan-Feb;15(1):016012. doi: 10.1117/1.3306704.
Abstract
We demonstrate the feasibility of fluorescence imaging of deeply seated tumors using mice injected with an angiogenesis tracer, a vascular endothelial growth factor conjugated with the infrared dye cyanine 7 (VEGF/Cy7). Our optical-only imaging reconstruction method separately estimates the target depth, and then applies this information to reconstruct functional information such as fluorophore concentration. Fluorescence targets with concentrations as low as sub-25 nM are well reconstructed at depths up to 2 cm in both homogeneous and heterogeneous media with this technique.
摘要
我们使用注射了血管生成示踪剂（一种与近红外染料 Cy7 偶联的血管内皮生长因子）的小鼠，证明了对深部肿瘤进行荧光成像的可行性。我们的仅光学成像重建方法分别估计目标深度，然后应用此信息来重建荧光团浓度等功能信息。使用该技术，在均匀和非均匀介质中，深度高达 2 厘米的荧光靶标，即使浓度低至亚 25 nM 也能得到很好的重建。
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