利用时门控荧光断层成像系统对体内光动力治疗光敏剂进行成像。
Imaging a photodynamic therapy photosensitizer in vivo with a time-gated fluorescence tomography system.
机构信息
Roswell Park Cancer Institute, Department of Cell Stress Biology and PDT Center, Elm and Carlton Streets, Buffalo, New York, 14263, USA.
出版信息
J Biomed Opt. 2012 Jul;17(7):071306. doi: 10.1117/1.JBO.17.7.071306.
Abstract
We report the tomographic imaging of a photodynamic therapy (PDT) photosensitizer, 2-(1-hexyloxyethyl)-2-devinyl pyropheophorbide-a (HPPH) in vivo with time-domain fluorescence diffuse optical tomography (TD-FDOT). Simultaneous reconstruction of fluorescence yield and lifetime of HPPH was performed before and after PDT. The methodology was validated in phantom experiments, and depth-resolved in vivo imaging was achieved through simultaneous three-dimensional (3-D) mappings of fluorescence yield and lifetime contrasts. The tomographic images of a human head-and-neck xenograft in a mouse confirmed the preferential uptake and retention of HPPH by the tumor 24-h post-injection. HPPH-mediated PDT induced significant changes in fluorescence yield and lifetime. This pilot study demonstrates that TD-FDOT may be a good imaging modality for assessing photosensitizer distributions in deep tissue during PDT monitoring.
摘要
我们利用时域荧光漫射光学断层成像术（TD-FDOT）报告了光动力疗法（PDT）光敏剂 2-（1-己氧基乙基）-2-去乙烯基焦脱镁叶绿酸-a（HPPH）的体内层析成像。在 PDT 前后进行了 HPPH 的荧光产率和寿命的同时重建。该方法在体模实验中得到了验证，并通过荧光产率和寿命对比度的同时三维（3-D）映射实现了深度分辨的体内成像。在小鼠的人头颈部异种移植模型中的层析成像证实了 HPPH 在注射后 24 小时内被肿瘤优先摄取和保留。HPPH 介导的 PDT 引起了荧光产率和寿命的显著变化。这项初步研究表明，TD-FDOT 可能是一种很好的成像方式，可用于评估 PDT 监测期间深部组织中光敏剂的分布。
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