University of California at Davis, Department of Physics, Davis, California 95616, USA.
J Biomed Opt. 2010 Jan-Feb;15(1):016018. doi: 10.1117/1.3309743.
We describe a high-throughput scanning optical microscope for detecting small-molecule compound microarrays on functionalized glass slides. It is based on measurements of oblique-incidence reflectivity difference and employs a combination of a y-scan galvometer mirror and an x-scan translation stage with an effective field of view of 2 cm x 4 cm. Such a field of view can accommodate a printed small-molecule compound microarray with as many as 10,000 to 20,000 targets. The scanning microscope is capable of measuring kinetics as well as endpoints of protein-ligand reactions simultaneously. We present the experimental results on solution-phase protein reactions with small-molecule compound microarrays synthesized from one-bead, one-compound combinatorial chemistry and immobilized on a streptavidin-functionalized glass slide.
我们描述了一种用于检测功能化玻璃载玻片上小分子化合物微阵列的高通量扫描光学显微镜。它基于斜入射反射率差异的测量，并采用 y 扫描振镜和 x 扫描平移台的组合，有效视场为 2 cm x 4 cm。这样的视场可以容纳多达 10,000 到 20,000 个目标的打印小分子化合物微阵列。扫描显微镜能够同时测量蛋白质-配体反应的动力学和终点。我们展示了从小分子化合物微阵列中进行溶液相蛋白质反应的实验结果，这些微阵列是由单珠、单化合物组合化学合成并固定在链霉亲和素功能化的玻璃载玻片上的。
