Anna University Chennai, Department of Physics, Division of Medical Physics and Lasers, Chennai, India.
J Biomed Opt. 2010 Jan-Feb;15(1):017010. doi: 10.1117/1.3324771.
Native fluorescence spectroscopy has shown potential to characterize and diagnose oral malignancy. We aim at extending the native fluorescence spectroscopy technique to characterize normal and oral submucous fibrosis (OSF) patients under pre- and post-treated conditions, and verify whether this method could also be considered in the monitoring of therapeutic prognosis noninvasively. In this study, 28 normal subjects and 28 clinically proven cases of OSF in the age group of 20 to 40 years are diagnosed using native fluorescence spectroscopy. The OSF patients are given dexamethasone sodium phosphate and hyaluronidase twice a week for 6 weeks, and the therapeutic response is monitored using fluorescence spectroscopy. The fluorescence emission spectra of normal and OSF cases of both pre- and post-treated conditions are recorded in the wavelength region of 350 to 600 nm at an excitation wavelength of 330 nm. The statistical significance is verified using discriminant analysis. The oxidation-reduction ratio of the tissue is also calculated using the fluorescence emission intensities of flavin adenine dinucleotide and nicotinamide adinine dinucleotide at 530 and 440 nm, respectively, and they are compared with conventional physical clinical examinations. This study suggests that native fluorescence spectroscopy could also be extended to OSF diagnosis and therapeutic prognosis.
自发荧光光谱学已显示出用于表征和诊断口腔恶性肿瘤的潜力。我们旨在扩展自发荧光光谱技术，以表征正常和口腔黏膜下纤维化（OSF）患者的预处理和后处理条件，并验证该方法是否也可用于无创监测治疗预后。在这项研究中，使用自发荧光光谱学诊断了 28 名年龄在 20 岁至 40 岁之间的正常受试者和 28 名经临床证实的 OSF 病例。将磷酸地塞米松钠和透明质酸酶每周两次给予 OSF 患者，共 6 周，并使用荧光光谱法监测治疗反应。在激发波长为 330nm 的情况下，记录了预处理和后处理条件下正常和 OSF 病例的 350nm 至 600nm 波长范围内的荧光发射光谱。使用判别分析验证了统计学意义。还使用黄素腺嘌呤二核苷酸和烟酰胺腺嘌呤二核苷酸在 530nm 和 440nm 处的荧光发射强度分别计算组织的氧化还原比，并将其与常规物理临床检查进行比较。本研究表明，自发荧光光谱学也可扩展用于 OSF 诊断和治疗预后。
