O'Reilly K L, Splitter G A
Department of Veterinary Science, University of Wisconsin-Madison 53706.
Vet Immunol Immunopathol. 1991 Jan;27(1-3):231-4. doi: 10.1016/0165-2427(91)90105-l.
Non-T and non-B lymphocytes (null cells) were obtained by Sephadex G-10 depletion followed by treatment with mAbs to CD2 and MHC class II and complement. The enriched cells were principally CD5dim and contained greatly increased numbers of null lymphocytes. This methodology will be useful for null lymphocyte enrichment in examining cell surface molecules and functional attributes of null lymphocytes.
通过葡聚糖凝胶G-10去除法，随后用抗CD2和MHC II类单克隆抗体及补体处理，获得非T和非B淋巴细胞（裸细胞）。富集的细胞主要为CD5dim，且裸淋巴细胞数量大幅增加。这种方法将有助于在研究裸淋巴细胞的细胞表面分子和功能特性时富集裸淋巴细胞。
