剪接体小核 RNA 修饰及其功能。
Spliceosomal snRNA modifications and their function.
机构信息
Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, NY, USA.
出版信息
RNA Biol. 2010 Mar-Apr;7(2):192-204. doi: 10.4161/rna.7.2.11207. Epub 2010 Mar 14.
Abstract
Spliceosomal snRNAs are extensively 2'-O-methylated and pseudouridylated. The modified nucleotides are relatively highly conserved across species, and are often clustered in regions of functional importance in pre-mRNA splicing. Over the past decade, the study of the mechanisms and functions of spliceosomal snRNA modifications has intensified. Two independent mechanisms behind these modifications, RNA-independent (protein-only) and RNA-dependent (RNA-guided), have been discovered. The role of spliceosomal snRNA modifications in snRNP biogenesis and spliceosome assembly has also been verified.
摘要
剪接体 snRNA 广泛地 2'-O-甲基化和假尿嘧啶化。修饰核苷酸在物种间相对高度保守，并且经常聚集在 pre-mRNA 剪接中具有功能重要性的区域。在过去的十年中，对剪接体 snRNA 修饰的机制和功能的研究已经加强。已经发现了这两种修饰背后的两种独立机制，即 RNA 非依赖性（仅蛋白）和 RNA 依赖性（RNA 引导）。剪接体 snRNA 修饰在 snRNP 生物发生和剪接体组装中的作用也得到了验证。
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