从外耳道炎分离出的念珠菌属的蛋白酶活性：初步结果。

Protease activities of Candida spp. isolated from otitis externa: preliminary result.

作者信息

Arsović N A, Banko A V, Dimitrijević M V, Djordjević V Z, Milovanović J P, Arsenijević V A

机构信息

Institute of Otorhinolaryngology and Maxillofacial Surgery, School of Medicine, University of Belgrade, Clinical Center of Serbia, Belgrade, Serbia.

出版信息

Acta Chir Iugosl. 2009;56(3):113-6. doi: 10.2298/aci0903113a.

DOI:10.2298/aci0903113a

PMID:20218114

Abstract

Otomycosis is a fungal infection of the ear predominantly caused by Candida and Aspergillus spp. The possible virulence factors of Candida spp. are enzymes, such as proteases, phospholipases, phosphatases and esterase. According to our knowledge, protease production in Candida strains isolated from patients with otomycosis has not been investigated. The present study was aimed at determining in vitro protease activity in 8 strains of Candida spp. (C. parapsilosis, C. famata, C. guilliermondii and C. albicans) isolated from children with otomycosis. A majority of isolated strains 7/8 (87.5%) were protease positive. The protease activity ranged from Pz 0.61 to 0.78. Further investigation is necessary to clarify the contribution of protease production to Candida virulence associated with otomycosis.

摘要

耳霉菌病是一种主要由念珠菌属和曲霉菌属引起的耳部真菌感染。念珠菌属可能的毒力因子是酶，如蛋白酶、磷脂酶、磷酸酶和酯酶。据我们所知，从患有耳霉菌病的患者中分离出的念珠菌菌株产生蛋白酶的情况尚未得到研究。本研究旨在测定从患有耳霉菌病的儿童中分离出的8株念珠菌属菌株（近平滑念珠菌、法塔念珠菌、季也蒙念珠菌和白色念珠菌）的体外蛋白酶活性。大多数分离菌株8株中有7株（87.5%）蛋白酶呈阳性。蛋白酶活性范围为Pz 0.61至0.78。有必要进一步研究以阐明蛋白酶产生对与耳霉菌病相关的念珠菌毒力的作用。