Ramos Lívia de Souza, Barbedo Leonardo Silva, Braga-Silva Lys Adriana, dos Santos André Luis Souza, Pinto Marcia Ribeiro, Sgarbi Diana Bridon da Graça
Instituto Biomédico, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil; Instituto de Microbiologia Paulo de Góes and Programa de Pós-Graduação em Bioquímica - Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
Rev Iberoam Micol. 2015 Apr-Jun;32(2):122-5. doi: 10.1016/j.riam.2014.01.003. Epub 2014 May 20.
Cases of superficial and invasive mycoses caused by emerging species of Candida have been increasingly reported over the last thirty years. The production of hydrolytic enzymes plays a central role in the fungal infective process. In Candida infections the secretion of both proteases and phospholipases are well-known virulence attributes.
To determine the protease and phospholipase production from 58 human clinical isolates of Candida obtained from individuals with cutaneous candidiasis seen in the Human and Veterinary Diagnostic Mycology Sector from Universidade Federal Fluminense (UFF), Brazil, from November 2008 to August 2009.
Fungal identification was performed using biochemical tests. Proteolytic activity was detected on agar plates containing bovine serum albumin, and phospholipase production was determined on egg-yolk plates.
The Candida species isolated were Candida parapsilosis (27.59%), Candida famata (18.96%), Candida albicans (15.52%), Candida haemulonii (12.06%), Candida ciferri (8.62%), Candida guilliermondii (6.90%), Candida tropicalis (5.17%) and Candida lipolytica (5.17%). All isolates of C. albicans produced both protease and phospholipase. As regards the isolates of non-C. albicans Candida species, 53.06% and 4.08% were able to produce protease and phospholipase, respectively. For example, the majority of isolates of C. parapsilosis (15/16) produced protease, while 40% of C. ciferri isolates (2/5) were phospholipase producers. This study shows, for the first time, that C. ciferri and C. haemulonii strains were able to produce protease.
Collectively, our results showed that different species of Candida isolated from cutaneous lesions were able to produce proteases and/or phospholipases, which are multifunctional molecules directly involved in the infectious process of these fungi.
在过去三十年中，由新型念珠菌引起的浅表和侵袭性真菌病病例报告日益增多。水解酶的产生在真菌感染过程中起着核心作用。在念珠菌感染中，蛋白酶和磷脂酶的分泌都是众所周知的毒力属性。
确定2008年11月至2009年8月从巴西弗卢米嫩塞联邦大学（UFF）人类和兽医诊断真菌学部门的皮肤念珠菌病患者中分离出的58株人类临床念珠菌菌株的蛋白酶和磷脂酶产生情况。
使用生化试验进行真菌鉴定。在含有牛血清白蛋白的琼脂平板上检测蛋白水解活性，在蛋黄平板上测定磷脂酶产生情况。
分离出的念珠菌种类有近平滑念珠菌（27.59%）、法塔念珠菌（18.96%）、白色念珠菌（15.52%）、哈氏念珠菌（12.06%）、西弗念珠菌（8.62%）、季也蒙念珠菌（6.90%）、热带念珠菌（5.17%）和解脂念珠菌（5.17%）。所有白色念珠菌分离株均产生蛋白酶和磷脂酶。至于非白色念珠菌的分离株，分别有53.06%和4.08%能够产生蛋白酶和磷脂酶。例如，大多数近平滑念珠菌分离株（15/16）产生蛋白酶，而40%的西弗念珠菌分离株（2/5）产生磷脂酶。本研究首次表明，西弗念珠菌和哈氏念珠菌菌株能够产生蛋白酶。
总体而言，我们的结果表明，从皮肤病变中分离出的不同念珠菌种类能够产生蛋白酶和/或磷脂酶，这些多功能分子直接参与这些真菌的感染过程。
