Dorko E, Jenca A, Orencák M, Virágová S, Pilipcinec E
Department of Epidemiology, Faculty of Medicine, Safárik University, 041 80 Kosice, Slovakia.
Folia Microbiol (Praha). 2004;49(5):601-4. doi: 10.1007/BF02931541.
Mycological analysis of swabs and scraping samples from the external ear canals of 40 patients with clinically diagnosed otomycosis (10 neonates, 30 adults) revealed the presence of fungi as etiological agents. They were investigated microscopically using 20 % potassium hydroxide, and by cultivation on Sabouraud's glucose agar. The Candida species were identified using the germ-tube test, micromorphology observations of colonies on rice agar, and particularly by the commercial kit AUXAcolor. The following Candida species were identified in the aural material examined: C. albicans (n = 21; 52.5 %), C. parapsilosis (11; 27.5), C. tropicalis (3; 7.5), C. krusei (3; 7.5), C. guilliermondii (2; 5.0). The above yeasts were present in samples together with Staphylococcus epidermidis (31), S. aureus (16), alpha-hemolytic streptococci (14), Neisseria spp. (14), Proteus mirabilis (3), Pseudomonas aeruginosa (3), Escherichia coli (1) and Haemophilus influenzae (1). The most frequent predisposing factors for otomycosis were swimming in public pools and/or bath, spa and diabetes mellitus.
对40例临床诊断为外耳道真菌病的患者(10例新生儿,30例成人)的外耳道拭子和刮片样本进行真菌学分析,结果显示存在真菌病原体。使用20%氢氧化钾对样本进行显微镜检查,并在沙氏葡萄糖琼脂培养基上进行培养。利用芽管试验、在米琼脂上观察菌落的微观形态,特别是使用商业试剂盒AUXAcolor来鉴定念珠菌属。在所检查的耳部材料中鉴定出以下念珠菌属:白色念珠菌(n = 21;52.5%)、近平滑念珠菌(11;27.5%)、热带念珠菌(3;7.5%)、克柔念珠菌(3;7.5%)、季也蒙念珠菌(2;5.0%)。上述酵母菌与表皮葡萄球菌(31例)、金黄色葡萄球菌(16例)、α-溶血性链球菌(14例)、奈瑟菌属(14例)、奇异变形杆菌(3例)、铜绿假单胞菌(3例)、大肠埃希菌(1例)和流感嗜血杆菌(1例)一起存在于样本中。外耳道真菌病最常见的诱发因素是在公共泳池和/或浴室、温泉浴场游泳以及糖尿病。