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分析小鼠和人体内生育酚和生育三烯醇的多种代谢物。

Analysis of multiple metabolites of tocopherols and tocotrienols in mice and humans.

机构信息

Department of Chemical Biology, Center for Cancer Prevention Research, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, New Jersey 08854, USA.

出版信息

J Agric Food Chem. 2010 Apr 28;58(8):4844-52. doi: 10.1021/jf904464u.

DOI:10.1021/jf904464u
PMID:20222730
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2858244/
Abstract

Tocopherols and tocotrienols, collectively known as vitamin E, are essential antioxidant nutrients. The biological fates and metabolite profiles of the different forms are not clearly understood. The objective of this study is to simultaneously analyze the metabolites of different tocopherols and tocotrienols in mouse and human samples. Using HPLC/electrochemical detection and mass spectrometry, 18 tocopherol-derived and 24 tocotrienol-derived side-chain degradation metabolites were identified in fecal samples. Short-chain degradation metabolites, in particular gamma- and delta-carboxyethyl hydroxychromans (CEHCs) and carboxymethylbutyl hydroxychromans (CMBHCs) were detected in urine, serum, and liver samples, with tocopherols additionally detected in serum and liver samples. The metabolite profiles of tocotrienols and tocopherols were similar, but new tocotrienol metabolites with double bonds were identified. This is the first comprehensive report describing simultaneous analysis of different side-chain metabolites of tocopherols and tocotrienols in mice and humans. Urinary metabolites may serve as useful biomarkers for the nutritional assessment of vitamin E.

摘要

生育酚和三烯生育酚统称为维生素 E，是必需的抗氧化营养素。不同形式的生物命运和代谢物特征尚未明确。本研究旨在同时分析小鼠和人粪便样品中不同生育酚和三烯生育酚的代谢物。利用高效液相色谱/电化学检测和质谱法，在粪便样品中鉴定出 18 种生育酚衍生的和 24 种三烯生育酚衍生的侧链降解代谢物。短链降解代谢物，特别是γ-和δ-羧乙基羟色烷（CEHCs）和羧甲基丁基羟色烷（CMBHCs），在尿液、血清和肝脏样品中检测到，血清和肝脏样品中还检测到生育酚。生育酚和三烯生育酚的代谢物谱相似，但鉴定出了具有双键的新三烯生育酚代谢物。这是首次全面描述在小鼠和人类中同时分析不同侧链生育酚和三烯生育酚代谢物的报告。尿代谢物可能是维生素 E 营养评估的有用生物标志物。

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