Fénichel P, Donzeau M, Farahifar D, Basteris B, Ayraud N, Hsi B L
Institut National de la Santé et de la Recherche Médicale (INSERM U210), Faculté de Médecine de Nice, France.
Fertil Steril. 1991 May;55(5):994-9. doi: 10.1016/s0015-0282(16)54312-x.
Acrosomal status has been studied on human sperm prepared for in vitro fertilization (IVF) and related to the rate of fertilization.
A group of 41 men with normal classical semen parameters, included in the IVF program of University of Nice for feminine tubal obstruction (n = 37) or unexplained infertility (n = 4), were evaluated in a prospective study and compared with a control group of 10 fertile donors.
Evaluation of acrosome status (spontaneous and A23187-induced acrosome loss) after 6 hours incubation in Ménézo's B2 medium was made by flow cytometry on suspended cells with a new immunofluorescence test recently reported by the authors based on a monoclonal antibody GB24.
Spontaneous acrosome loss remained low even after 6 hours capacitation (mean + 1 SD, 6.5% + 4.9%). Response to A23187 increased with the duration of preincubation with a marked response after 6 hours (29.5% + 8.9%). Low spontaneous acrosome loss (less than mean + 1 SD) and high response to A23187 (greater than mean - 1 SD) were observed in 25 out of 26 cases of group A with a high fertilization rate (greater than 50% fertilized oocytes). A high level of spontaneous acrosome loss and/or a lack of response to A23187 was observed in 2 of 7 cases of group B (fertilization rate less than 50%) and 6 of 8 cases of group C (unexplained unsuccessful fertilization).
Impaired acrosomal status can be associated with unexplained unsuccessful fertilization.
研究了为体外受精（IVF）准备的人类精子的顶体状态，并将其与受精率相关联。
一组41名经典精液参数正常的男性，因女性输卵管阻塞（n = 37）或不明原因不孕症（n = 4）纳入尼斯大学的IVF项目，在一项前瞻性研究中进行评估，并与10名有生育能力的供体组成的对照组进行比较。
在Ménézo's B2培养基中孵育6小时后，通过流式细胞术对悬浮细胞进行顶体状态评估（自发和A23187诱导的顶体丢失），该评估基于作者最近报道的一种基于单克隆抗体GB24的新免疫荧光试验。
即使在6小时的获能后，自发顶体丢失率仍然很低（平均值±1标准差，6.5%±4.9%）。对A23187的反应随预孵育时间的延长而增加，6小时后有明显反应（29.5%±8.9%）。在受精率高（大于50%的受精卵）的A组26例中的25例中观察到低自发顶体丢失（小于平均值±1标准差）和对A23187的高反应（大于平均值-1标准差）。在受精率低（小于50%）的B组7例中的2例以及C组（不明原因的受精失败）8例中的6例中观察到高水平的自发顶体丢失和/或对A23187无反应。
顶体状态受损可能与不明原因的受精失败有关。
