强毒金黄色葡萄球菌噬菌体SA97的分离与基因组特征分析
Isolation and Genome Characterization of the Virulent Staphylococcus aureus Bacteriophage SA97.
作者信息
Chang Yoonjee, Shin Hakdong, Lee Ju-Hoon, Park Chul Jong, Paik Soon-Young, Ryu Sangryeol
机构信息
Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, and Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, Korea.
Department of Food Science and Biotechnology, Kyung Hee University, Yongin 446-701, Korea.
出版信息
Viruses. 2015 Oct 1;7(10):5225-42. doi: 10.3390/v7102870.
A novel bacteriophage that infects S. aureus, SA97, was isolated and characterized. The phage SA97 belongs to the Siphoviridae family, and the cell wall teichoic acid (WTA) was found to be a host receptor of the phage SA97. Genome analysis revealed that SA97 contains 40,592 bp of DNA encoding 54 predicted open reading frames (ORFs), and none of these genes were related to virulence or drug resistance. Although a few genes associated with lysogen formation were detected in the phage SA97 genome, the phage SA97 produced neither lysogen nor transductant in S. aureus. These results suggest that the phage SA97 may be a promising candidate for controlling S. aureus.
一种新型的感染金黄色葡萄球菌的噬菌体SA97被分离并进行了表征。噬菌体SA97属于长尾噬菌体科，并且发现细胞壁磷壁酸（WTA）是噬菌体SA97的宿主受体。基因组分析表明，SA97含有40,592 bp的DNA，编码54个预测的开放阅读框（ORF），并且这些基因均与毒力或耐药性无关。尽管在噬菌体SA97基因组中检测到了一些与溶原形成相关的基因，但噬菌体SA97在金黄色葡萄球菌中既不产生溶原菌也不产生转导子。这些结果表明，噬菌体SA97可能是控制金黄色葡萄球菌的一个有前途的候选者。
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