Faculty of Pharmacy, Pharmacognosy Department, Charles University in Prague, Heyrovského 1203, 500 05, Hradec Králové, Czech Republic.
Biotechnol Lett. 2010 Jul;32(7):935-41. doi: 10.1007/s10529-010-0225-9. Epub 2010 Mar 13.
Cannabis sativa L. plants produce a diverse array of secondary metabolites. Cannabis cell cultures were treated with jasmonic acid (JA) and pectin as elicitors to evaluate their effect on metabolism from two cell lines using NMR spectroscopy and multivariate data analysis. According to principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA), the chloroform extract of the pectin-treated cultures were more different than control and JA-treated cultures; but in the methanol/water extract the metabolome of the JA-treated cells showed clear differences with control and pectin-treated cultures. Tyrosol, an antioxidant metabolite, was detected in cannabis cell cultures. The tyrosol content increased after eliciting with JA.
大麻属植物产生多种次生代谢产物。用茉莉酸（JA）和果胶作为诱导子处理大麻细胞培养物，并用 NMR 光谱和多元数据分析评估它们对两种细胞系代谢的影响。根据主成分分析（PCA）和偏最小二乘判别分析（PLS-DA），果胶处理培养物的氯仿提取物与对照和 JA 处理培养物的差异更大；但在甲醇/水提取物中，JA 处理细胞的代谢组与对照和果胶处理培养物的差异明显。在大麻细胞培养物中检测到抗氧化代谢物酪醇。用 JA 诱导后，酪醇含量增加。
