Linhart S B, Blom F S, Dasch G J, Roberts J D, Engeman R M, Esposito J J, Shaddock J H, Baer G M
Denver Wildlife Research Center, Animal and Plant Health Inspection Service, Colorado 80225.
J Wildl Dis. 1991 Jan;27(1):21-33. doi: 10.7589/0090-3558-27.1.21.
Captive raccoons were offered a variety of vaccine containers and bait components in a series of three-choice tests. Paraffin wax ampules were the most readily accepted vaccine container. Preferred bait components included corn and shellfish oils, deep fried corn meal batter, and egg, apple and buttermilk flavorings. These results, together with factors including ease of bait formulation, cost, and suitability for field use, were used to develop an experimental delivery system for an oral rabies vaccine. The developed system was composed of a polyurethane sleeve (1.5 x 5.5 cm) dipped in a commercial food batter mix together with corn meal, milk and egg. The sleeve was deep fried in corn oil and a 2.0 ml ampule containing a recombinant rabies vaccine was then inserted into the sleeve bait. These baits were presented to 10 captive raccoons. Nine of the 10 animals developed high levels of rabies virus neutralizing antibodies. Field tests are needed to determine if the delivery system developed also is effective for wild raccoons.
在一系列三选测试中，向圈养的浣熊提供了多种疫苗容器和诱饵成分。石蜡蜡安瓿是最容易被接受的疫苗容器。首选的诱饵成分包括玉米油和贝类油、油炸玉米面糊以及鸡蛋、苹果和酪乳调味料。这些结果，连同诱饵配制的难易程度、成本和野外使用的适用性等因素，被用于开发一种口服狂犬病疫苗的实验性递送系统。所开发的系统由一个聚氨酯套管（1.5×5.5厘米）组成，该套管浸在一种商业食品面糊混合物中，再加上玉米粉、牛奶和鸡蛋。将套管在玉米油中油炸，然后将一个装有重组狂犬病疫苗的2.0毫升安瓿插入套管诱饵中。将这些诱饵提供给10只圈养的浣熊。10只动物中有9只产生了高水平的狂犬病病毒中和抗体。需要进行野外测试以确定所开发的递送系统对野生浣熊是否也有效。
