一种在大肠杆菌中将多种非天然氨基酸基因掺入一种蛋白质的简便方法。
A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli.
作者信息
Huang Ying, Russell William K, Wan Wei, Pai Pei-Jing, Russell David H, Liu Wenshe
机构信息
Department of Chemistry, Texas A&M University, College Station, TX, 77843, USA.
出版信息
Mol Biosyst. 2010 Apr;6(4):683-6. doi: 10.1039/b920120c. Epub 2010 Feb 15.
DOI:10.1039/b920120c
PMID:20237646
Abstract
By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three N(epsilon)-acetyl-l-lysines into one GFP(UV) protein in E. coli.
摘要
通过过表达核糖体蛋白L11的C末端结构域以减少释放因子1介导的蛋白质翻译终止，在大肠杆菌中实现了增强的琥珀色抑制。这种增强的琥珀色抑制效率使得在大肠杆菌中能够将三个N（ε）-乙酰基-L-赖氨酸基因掺入一个绿色荧光蛋白（UV）中。
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