Huang Ying, Russell William K, Wan Wei, Pai Pei-Jing, Russell David H, Liu Wenshe
Department of Chemistry, Texas A&M University, College Station, TX, 77843, USA.
Mol Biosyst. 2010 Apr;6(4):683-6. doi: 10.1039/b920120c. Epub 2010 Feb 15.
By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three N(epsilon)-acetyl-l-lysines into one GFP(UV) protein in E. coli.
通过过表达核糖体蛋白L11的C末端结构域以减少释放因子1介导的蛋白质翻译终止，在大肠杆菌中实现了增强的琥珀色抑制。这种增强的琥珀色抑制效率使得在大肠杆菌中能够将三个N（ε）-乙酰基-L-赖氨酸基因掺入一个绿色荧光蛋白（UV）中。
