Byrom B, Yunker C E, Donovan P L, Smith G E
University of Florida.
Vet Microbiol. 1991 Feb 1;26(3):263-8. doi: 10.1016/0378-1135(91)90019-c.
A new and simple technique for isolation of C. ruminantium in bovine and ovine vascular endothelial cells (aorta, pulmonary artery) is described. Unlike previous studies, no efforts were made to retard cell growth by irradiation or chemicals. Instead, heparin-derived plasma samples obtained from only those animals exhibiting prolonged or extremely high temperatures were used. In this way, C. ruminantium was isolated from 27 of 37 samples (73%) and from 22 of 26 animals (85%). A total of six Zimbabwean stocks of C. ruminantium were isolated in cell culture.
本文描述了一种从牛和羊的血管内皮细胞（主动脉、肺动脉）中分离反刍兽类无形体的新的简单技术。与以往的研究不同，本研究未通过辐射或化学物质来抑制细胞生长。相反，仅使用从那些体温持续升高或极高的动物身上采集的肝素化血浆样本。通过这种方法，从37个样本中的27个（73%）以及26只动物中的22只（85%）中分离出了反刍兽类无形体。在细胞培养中总共分离出了6株津巴布韦反刍兽类无形体毒株。
