Juvonen P, Jakobsson I, Lindberg T
Department of Paediatrics, University of Lund, Malmö General Hospital, Sweden.
Arch Dis Child. 1991 Mar;66(3):300-3. doi: 10.1136/adc.66.3.300.
The absorption of macromolecules was analysed by measuring serum concentrations of human alpha-lactalbumin after feeding human milk, using a competitive radioimmunoassay. The control group consisted of 78 children fed by cows' milk formula; concentrations of alpha-lactalbumin in their serum were low. The median concentrations in the different age groups varied between 7-13 micrograms alpha-lactalbumin/1 serum/1 human milk/kg body weight. Twenty-eight children with cows' milk allergy were studied before introduction of a diet free of cows' milk. Nineteen had gastrointestinal and nine skin symptoms. High serum alpha-lactalbumin concentrations were found. Only two children had alpha-lactalbumin concentrations below 100 micrograms alpha-lactalbumin/1 serum/1 human milk/kg body weight. Altogether 76 children on a diet free of cows' milk were studied directly after a cows' milk challenge; 26 developed symptoms. They had significantly higher serum alpha-lactalbumin values than the 50 children with no symptoms upon challenge. Eighteen of the 26 children with symptoms had serum alpha-lactalbumin concentrations of more than 100 micrograms/1 serum/1 human milk/kg body weight in contrast with two of the 50 with no symptoms. Total serum IgE antibodies were analysed in 43 children. No correlation was found between concentrations of serum IgE and serum alpha-lactalbumin in the children with cows' milk allergy.
采用竞争性放射免疫分析法，通过测量喂食人乳后血清中人α-乳白蛋白的浓度，分析大分子的吸收情况。对照组由78名喂食牛奶配方奶粉的儿童组成；他们血清中的α-乳白蛋白浓度较低。不同年龄组的中位数浓度在7 - 13微克α-乳白蛋白/1血清/1人乳/千克体重之间。对28名牛奶过敏儿童在引入无牛奶饮食前进行了研究。其中19名有胃肠道症状，9名有皮肤症状。发现血清α-乳白蛋白浓度较高。只有两名儿童的α-乳白蛋白浓度低于100微克α-乳白蛋白/1血清/1人乳/千克体重。共有76名无牛奶饮食的儿童在牛奶激发试验后直接接受研究；其中26名出现症状。他们血清α-乳白蛋白值显著高于激发试验后无症状的50名儿童。出现症状的26名儿童中有18名血清α-乳白蛋白浓度超过100微克/1血清/1人乳/千克体重，而无症状的50名儿童中只有两名如此。对43名儿童分析了血清总IgE抗体。在牛奶过敏儿童中，未发现血清IgE浓度与血清α-乳白蛋白之间存在相关性。
