循环免疫复合物中特异性抗原的检测：该检测方法的验证及其在健康和食物过敏受试者中形成的食物抗原-抗体复合物的应用。
Detection of specific antigen within circulating immune complexes: validation of the assay and its application to food antigen-antibody complexes formed in healthy and food-allergic subjects.
作者信息
Paganelli R, Levinsky R J, Atherton D J
出版信息
Clin Exp Immunol. 1981 Oct;46(1):44-53.
Abstract
A simple two-step method for the detection of specific antigen within immune complexes is described. The immune complexes are precipitated from serum by polyethylene glycol, dissociated by incubation in acid pH buffer and adsorbed onto the surface of polystyrene tubes. The antigen is detected by the binding of a radiolabelled affinity-purified specific antibody. The assay can detect the antigen within both antigen- and antibody-excess immune complexes of any immunoglobulin class, and can also allow semiquantitative comparison of different samples. Immune complexes containing food protein antigens after eating have been found in the serum of both normal subjects and atopic patients; the latter group showed higher mean levels of antigen-specific immune complexes. The method can be adopted for large-scale screening of clinical samples for suspected antigens if suitable antisera are available.
摘要
本文描述了一种用于检测免疫复合物中特异性抗原的简单两步法。免疫复合物通过聚乙二醇从血清中沉淀出来，在酸性pH缓冲液中孵育使其解离，然后吸附到聚苯乙烯管表面。通过放射性标记的亲和纯化特异性抗体的结合来检测抗原。该检测方法可以检测任何免疫球蛋白类别的抗原过量和抗体过量免疫复合物中的抗原，还可以对不同样本进行半定量比较。在正常受试者和特应性患者的血清中都发现了进食后含有食物蛋白抗原的免疫复合物；后一组的抗原特异性免疫复合物平均水平较高。如果有合适的抗血清，该方法可用于大规模筛查临床样本中的可疑抗原。
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