通过膜色谱法浓缩和纯化肠道病毒。
Concentration and purification of enteroviruses by membrane chromatography.
作者信息
Henderson M, Wallis C, Melnick J L
出版信息
Appl Environ Microbiol. 1976 Nov;32(5):689-93. doi: 10.1128/aem.32.5.689-693.1976.
Abstract
A simple procedure for the concentration and partial purification of enteroviruses from tissue culture harvests is described. After removal of acid-precipitating components with a cationic detergent, the detergent and most membrane-coating components were removed by treatment with a cationic-exchange resin. The resin effluent was then acidified, and the virus was adsorbed to epoxy-fiberglass membranes. Virus was then eluted with pH 11.5 glycine-NaOH buffer. Since this eluate contains no orgcentrated simply by acidifying the eluate and passing it through a smaller membrane than that used for the first concentration. As high as 500-fold concentrations can be achieved, with a high efficiency of recovery.
摘要
本文描述了一种从组织培养收获物中浓缩和部分纯化肠道病毒的简单方法。用阳离子去污剂去除酸沉淀成分后，通过用阳离子交换树脂处理去除去污剂和大多数膜包被成分。然后将树脂流出液酸化，病毒吸附到环氧玻璃纤维膜上。接着用pH 11.5的甘氨酸 - 氢氧化钠缓冲液洗脱病毒。由于该洗脱液不含有机成分，只需将洗脱液酸化并通过比第一次浓缩所用膜更小的膜即可简单地进行浓缩。可实现高达500倍的浓缩，且回收率高。
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