Armon R, Neeman I, Kott Y
Department of Food Engineering, Israel Institute of Technology, Haifa.
J Med Virol. 1989 Dec;29(4):256-60. doi: 10.1002/jmv.1890290407.
A simple, rapid and efficient procedure of virus concentration from urine, cerebrospinal fluid (CSF), and feces was developed. Pig erythrocyte ghost cells were used to adsorb and elute such viruses as poliovirus LSC-1, echovirus 6, and human rotavirus (clinical isolate). In urine and CSF, the adsorption efficiency range was 80-100% and elution was from 85% to greater than 100%. In addition, poliovirus LSC-1 was used as an experimental model to examine this procedure under various clinical conditions, such as calcium, glucose, amino acids, and urea at high concentrations. These were added to normal urine specimens to simulate pathological conditions. The results suggest that pig erythrocyte ghost cells are an efficient matrix for adsorption and elution of enteric viruses found in clinical specimens of urine, CSF, and feces. This method might be useful in virus concentration from clinical specimens and for preparative microscopy and other clinical laboratory methods that require subsequent virus concentration.
开发了一种从尿液、脑脊液（CSF）和粪便中浓缩病毒的简单、快速且高效的方法。使用猪红细胞空壳细胞吸附和洗脱脊髓灰质炎病毒LSC-1、埃可病毒6和人轮状病毒（临床分离株）等病毒。在尿液和脑脊液中，吸附效率范围为80%-100%，洗脱率为85%至大于100%。此外，脊髓灰质炎病毒LSC-1被用作实验模型，在各种临床条件下，如高浓度的钙、葡萄糖、氨基酸和尿素，来检验该方法。将这些添加到正常尿液标本中以模拟病理状况。结果表明，猪红细胞空壳细胞是吸附和洗脱尿液、脑脊液和粪便临床标本中肠道病毒的有效基质。该方法可能有助于从临床标本中浓缩病毒，以及用于需要后续病毒浓缩的制备显微镜检查和其他临床实验室方法。
