Booth J C, Rweyemamu M M, Pay T W
J Hyg (Lond). 1978 Feb;80(1):31-42. doi: 10.1017/s0022172400053377.
Two-dimensional quantal microneutralization tests on foot-and-mouth disease viruses, in which neutralizing antibody activity was titrated against a serial range of virus doses, demonstrated a variety of dose-response curves some of which were rectilinear, others clearly curvilinear. Moreover, in the case of the non-linear responses obtained with some antisera, the shape of the curve was such that antibody titres recorded with doses of virus ranging from 10(3)-10(5) TCD50 were closely similar. Studies were carried out on the effect of varying the conditions of the test on the shape of the dose-response curve: significant differences were obtained after treatment of the antiserum-virus mixtures with anti-species globulin, and when the test was assayed in cells of differing susceptibility to infection.
针对口蹄疫病毒的二维定量微量中和试验，其中针对一系列连续的病毒剂量滴定中和抗体活性，显示出多种剂量反应曲线，其中一些是直线型的，另一些则明显是曲线型的。此外，在用某些抗血清获得非线性反应的情况下，曲线的形状使得用10(3)-10(5) TCD50的病毒剂量记录的抗体滴度非常相似。开展了关于改变试验条件对剂量反应曲线形状影响的研究：在用抗种属球蛋白处理抗血清-病毒混合物后，以及在对感染敏感性不同的细胞中进行试验时，均获得了显著差异。
