Huggett D O, Rodríguez J E, McKee A P
Infect Immun. 1972 Dec;6(6):996-1002. doi: 10.1128/iai.6.6.996-1002.1972.
Populations of reovirus type 1 could not be completely neutralized by exposure to specific antiviral serum. Aggregation of reovirions before or after exposure to neutralizing antibody was discarded as a possible explanation for the phenomenon observed. Anti-rabbit serum and equilibrium zonal density gradient centrifugation were used to show that the residual infectious fraction consisted of infectious antibody-virion complexes. Electron microscopy and equilibrium density gradient centrifugation of stock virus preparations were used to attempt to determine whether the infectious virus-antibody complexes resulted from the combination of antibody with virus particles which differed from neutralizable virions either morphologically or with regard to buoyant density. Although electron micrographs revealed the presence of three morphologically distinct classes of virus particles, density gradient centrifugation resulted in only one infectious peak. Density gradient centrifugation of virus exposed to antibody resulted in only one infectious peak which consisted of virus-antibody complexes.
1型呼肠孤病毒群体不能通过暴露于特异性抗病毒血清而被完全中和。在暴露于中和抗体之前或之后呼肠孤病毒的聚集被排除，不作为观察到的现象的一种可能解释。使用抗兔血清和平衡区带密度梯度离心法表明，残留的感染性部分由感染性抗体-病毒体复合物组成。利用电子显微镜和储备病毒制剂的平衡密度梯度离心法，试图确定感染性病毒-抗体复合物是否由抗体与在形态或浮力密度方面不同于可中和病毒体的病毒颗粒结合产生。尽管电子显微照片显示存在三种形态上不同的病毒颗粒类别，但密度梯度离心仅产生一个感染性峰。暴露于抗体的病毒进行密度梯度离心仅产生一个由病毒-抗体复合物组成的感染性峰。
