Hudson J B, Loh L, Misra V, Judd B, Suzuki J
J Gen Virol. 1978 Jan;38(1):149-59. doi: 10.1099/0022-1317-38-1-149.
Spleen cultures from various strains of mice were infected in vitro with murine cytomegalovirus (MCMV). Infectious centres were established in a small proportion (not greater than 1%) of the cells. Virus could be rescued from these cells by co-cultivation with syngeneic or allogeneic fibroblasts, but the frequency of rescue could not be altered by incubation with cyclic nucleotide analogues, iododeoxyuridine, cortisol, or allogeneic spleen cells. In addition a smaller fraction of the cell population, possibly a sub-population of the infectious centres, replicated virus spontaneously. The presence of mitogens did not affect these interactions qualitatively or quantitatively. A third response to infection was an inhibition in DNA synthesis, which was suffered by unstimulated cultures and by cells transformed by concanavalin A and bacterial lipopolysaccharides, although overall cell viability was maintained. This response was also mediated by u.v.-inactivated virus.
用鼠巨细胞病毒（MCMV）在体外感染来自不同品系小鼠的脾脏培养物。在一小部分（不超过1%）细胞中形成了感染中心。通过与同基因或异基因成纤维细胞共培养，可从这些细胞中拯救出病毒，但与环核苷酸类似物、碘脱氧尿苷、皮质醇或异基因脾细胞孵育并不能改变拯救频率。此外，细胞群体中的一小部分，可能是感染中心的一个亚群，会自发复制病毒。有丝分裂原的存在在质量或数量上均不影响这些相互作用。对感染的第三种反应是DNA合成受到抑制，未受刺激的培养物以及被伴刀豆球蛋白A和细菌脂多糖转化的细胞都会出现这种情况，尽管总体细胞活力得以维持。这种反应也由紫外线灭活的病毒介导。
