1型人类免疫缺陷病毒反义RNA靶区域的鉴定与分析
Identification and analysis of antisense RNA target regions of the human immunodeficiency virus type 1.
作者信息
Rittner K, Sczakiel G
机构信息
Institut für Virusforschung, Deutsches Krebsforschungszentrum, Heidelberg, FRG.
出版信息
Nucleic Acids Res. 1991 Apr 11;19(7):1421-6. doi: 10.1093/nar/19.7.1421.
Abstract
Antisense RNA, transcribed intracellularly from constitutive expression cassettes, inhibits the replication of the human immunodeficiency virus type 1 (HIV-1) as demonstrated by a quantitative microinjection assay in human SW480 cells. Infectious proviral HIV-1 DNA was co-microinjected together with a fivefold molar excess of plasmids expressing antisense RNA complementary to a set of ten different HIV-1 target regions. The most inhibitory antisense RNA expression plasmids were targeted against a 1 kb region within the gag open reading frame and against a 562 base region containing the coding sequences for the regulatory viral proteins tat and rev. Experimental evidence is presented that the antisense principle is the inhibitory mechanism in this assay system.
摘要
从组成型表达盒在细胞内转录的反义RNA可抑制1型人类免疫缺陷病毒（HIV-1）的复制，这在人SW480细胞的定量显微注射试验中得到了证实。将感染性前病毒HIV-1 DNA与五倍摩尔过量的表达与一组十个不同HIV-1靶区域互补的反义RNA的质粒共同显微注射。最具抑制性的反义RNA表达质粒靶向gag开放阅读框内的1 kb区域以及包含调节病毒蛋白tat和rev编码序列的562个碱基区域。本文提供的实验证据表明，反义原理是该检测系统中的抑制机制。
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