Miller Elzbieta, Mrowicka Małgorzata, Malinowska Katarzyna, Zołyński Krystian, Kedziora Józef
Rehabilitation Ward, III General Hospital in Lódz, Poland.
J Med Invest. 2010 Feb;57(1-2):168-73. doi: 10.2152/jmi.57.168.
There is evidence that multiple sclerosis (MS) is not only characterized by immune mediated inflammatory reactions but also by neurodegenerative processes. Neutralization of oxidative stress and excitotoxicity, might represent a therapeutic approach to provide neuroprotection in MS. The purpose of this study was to compare changes in total antioxidative status and activities of chosen antioxidative enzymes, such as: SOD, CAT in erythrocytes of patients with MS before and after using WBCT with control group.
32 patients with multiple sclerosis (ICD10-G35) and 20 healthy subjects were recruited for the study. The examined MS group (n=16) was treated with a series of 10 daily exposures in a cryogenic chamber (2-3 min, from -120 degrees C to -110 degrees C) and program of exercises. The control MS group (n=16) had only exercises. Plasma TAS as well as SOD and CAT activities in erythrocytes were measured.
The level of TAS in MS patients was distinctly reduced compared to healthy subjects. After two weeks of WBCT treatment an increase of TAS in the whole examined group (p>0.01) were observed in relation to control MS group. There was not increase of CuZnSOD and CAT activities.
Our results suggest positive antioxidant effects of WBCT as a short-term adjuvant treatment for patients suffered due to MS.
有证据表明，多发性硬化症（MS）不仅以免疫介导的炎症反应为特征，还具有神经退行性变过程。中和氧化应激和兴奋性毒性可能是一种在MS中提供神经保护的治疗方法。本研究的目的是比较使用全身冷冻疗法（WBCT）前后MS患者红细胞中总抗氧化状态和所选抗氧化酶（如超氧化物歧化酶（SOD）、过氧化氢酶（CAT））活性的变化，并与对照组进行比较。
招募了32例多发性硬化症（ICD10-G35）患者和20名健康受试者进行研究。接受检查的MS组（n = 16）在低温室接受一系列为期10天的治疗（2 - 3分钟，从-120摄氏度至-110摄氏度）并进行锻炼计划。对照MS组（n = 16）仅进行锻炼。测量血浆总抗氧化能力（TAS）以及红细胞中的SOD和CAT活性。
与健康受试者相比，MS患者的TAS水平明显降低。在WBCT治疗两周后，与对照MS组相比，整个检查组的TAS有所增加（p>0.01）。CuZnSOD和CAT活性没有增加。
我们的结果表明，WBCT作为对因MS患病患者的短期辅助治疗具有积极的抗氧化作用。
