Polatoglu Kaan, Demirci Fatih, Demirci Betül, Gören Nezhun, Başer Kemal Hüsnü Can
Department of Biology, 34210, Yildiz Technical University, Istanbul, Turkey.
J Oleo Sci. 2010;59(4):177-84. doi: 10.5650/jos.59.177.
Water-distilled essential oils from herbal parts of Tanacetum parthenium from two different localities in Turkey were analyzed by GC and GC/MS. The essential oil of T. parthenium collected from Davutpasa-Istanbul location were characterized with camphor 49%, trans-chrysanthenyl acetate 22.1% and camphene 9.4%. Second plant sample is collected from the remote east end of the country Savşat-Ardahan location. The essential oil from this location was characterized with camphor 60.8% and camphene 6.8%. Unlike the former this sample contains trans-chrysanthenyl acetate in trace amount and cis- chrysanthenyl acetate in very small amount (0.6%) which is not present in the first sample. Antibacterial activity of the oils were evaluated for five Gram (+) and five Gram (-) bacteria by using a broth microdilution assay. The highest activity was observed on Bacillus subtilis, Staphylococcus aureus and methicillin-resistant S.aureus however when compared with positive control oils showed higher MIC values. The oil of Istanbul-sample showed highest activity on B. subtilis (125 microg/mL) and methicillin-resistant S. aureus (125 microg/mL) which is two fold concentration when compared with the positive control chloramphenicol (62.5 microg/mL). The oil of Ardahan sample showed the highest activity on S. aureus (125 microg/mL) which is likewise two fold concentration of the positive control chloramphenicol (62.5 microg/mL). DPPH scavenging activity was 59.3% of the oil from Davutpasa at 15 mg/mL concentration. When compared to positive control alpha-tocopherol (94.6%) Savşat oil (28.2%) showed low and Davutpasa oil showed medium DPPH scavenging activity. All of the oils showed toxicity to Vibrio fischeri in the TLC-bioluminescence assay.
采用气相色谱（GC）和气相色谱-质谱联用（GC/MS）技术，对来自土耳其两个不同地区的艾菊全草经水蒸馏得到的精油进行了分析。从伊斯坦布尔达武特帕夏采集的艾菊精油中，樟脑含量为49%，反式菊烯醇乙酸酯含量为22.1%，莰烯含量为9.4%。第二个植物样本采集自该国偏远的东部地区萨夫萨特-阿尔达汉。该地区的精油中，樟脑含量为60.8%，莰烯含量为6.8%。与前者不同的是，该样本中反式菊烯醇乙酸酯含量微量，顺式菊烯醇乙酸酯含量极少（0.6%），而第一个样本中不存在顺式菊烯醇乙酸酯。采用肉汤微量稀释法，对这两种精油针对5种革兰氏阳性菌和5种革兰氏阴性菌的抗菌活性进行了评估。在枯草芽孢杆菌、金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌上观察到了最高活性，不过与阳性对照相比，精油显示出更高的最低抑菌浓度（MIC）值。伊斯坦布尔样本的精油对枯草芽孢杆菌（125微克/毫升）和耐甲氧西林金黄色葡萄球菌（125微克/毫升）表现出最高活性，与阳性对照氯霉素（62.5微克/毫升）相比，浓度高出两倍。阿尔达汉样本的精油对金黄色葡萄球菌（125微克/毫升）表现出最高活性，同样是阳性对照氯霉素（62.5微克/毫升）浓度的两倍。在浓度为15毫克/毫升时，达武特帕夏精油的二苯基苦味酰基自由基（DPPH）清除活性为59.3%。与阳性对照α-生育酚（94.6%）相比，萨夫萨特精油（28.2%）的DPPH清除活性较低，达武特帕夏精油的DPPH清除活性为中等。在薄层色谱-生物发光试验中，所有精油均对费氏弧菌显示出毒性。
