Garland S M, Locarnini S A, Gust I D
Pathology. 1979 Jul;11(3):393-9. doi: 10.3109/00313027909059017.
An enzyme-linked immunosorbent assay was established for detection of antibodies to rubella virus. In this system commercially available rubella antigen was attached to the wells of polystyrene microtitre plates after which sera were added and incubated to allow the formation of antigen-antibody complexes. The presence of bound antibody was detected by adding anti-human globulin coupled to horseradish peroxidase and visually observing the colour change produced after addition of an appropriate substrate. The test was reproducible and simple to perform and had a similar sensitivity to the widely used haemagglutination inhibition system.
建立了一种酶联免疫吸附测定法用于检测风疹病毒抗体。在该系统中，将市售风疹抗原附着于聚苯乙烯微量滴定板的孔中，然后加入血清并孵育，以使抗原 - 抗体复合物形成。通过加入与辣根过氧化物酶偶联的抗人球蛋白并目视观察加入适当底物后产生的颜色变化来检测结合抗体的存在。该试验可重复且操作简单，与广泛使用的血凝抑制系统具有相似的灵敏度。
