用酶联免疫吸附测定法检测风疹病毒抗体。
Detection of antibody to rubella virus by enzyme-linked immunosorbent assay.
作者信息
Gravell M, Dorsett P H, Gutenson O, Ley A C
出版信息
J Infect Dis. 1977 Oct;136 Suppl:S300-3. doi: 10.1093/infdis/136.supplement_2.s300.
Abstract
Titers of antibody to rubella virus in 68 human sera were compared by hemagglutination inhibition and enzyme-linked immunosorbent assay (ELISA). In general, the titers measured by ELISA were higher than those found by hemagglutination inhibition. Although the titers differed, the two methods showed parallel trends. Use of purified rubella virus and addition of 1% bovine serum albumin to the test wash solution aided in reducing false-positive titers in ELISA.
摘要
通过血凝抑制试验和酶联免疫吸附测定（ELISA）对68份人血清中抗风疹病毒抗体滴度进行了比较。总体而言，ELISA测定的滴度高于血凝抑制试验测得的滴度。尽管滴度有所不同，但两种方法呈现出平行趋势。使用纯化的风疹病毒并在检测洗涤液中添加1%的牛血清白蛋白有助于降低ELISA中的假阳性滴度。
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