基于神经母细胞瘤（Neuro-2a）细胞测定法检测和定量检测类似麦角毒素的化合物。在冈比甲藻属（ Gambierdiscus spp.）中类似麦角毒素化合物的筛选中的应用。
Detection and quantification of maitotoxin-like compounds using a neuroblastoma (Neuro-2a) cell based assay. Application to the screening of maitotoxin-like compounds in Gambierdiscus spp.
机构信息
IRTA, Sant Carles de la Ràpita, Ctra. Poble Nou s/n, 43540 Sant Carles de la Ràpita, Spain.
出版信息
Toxicon. 2010 Aug 1;56(1):36-44. doi: 10.1016/j.toxicon.2010.03.012. Epub 2010 Mar 21.
SK&F 96365 was used in a Neuroblastoma (Neuro-2a) cell based assay to determine the production of maitotoxin-like (MTX-like) compounds in two strains of Gambierdiscus spp. A 2.5 hour assay was effective for the detection of the MTX-induced toxic effects with a concentration that inhibited 50% cell viability (IC(50)) equivalent to 3.38 nM MTX. Evidence was found for the production of MTX-like compounds in both Gambierdiscus strains studied at concentrations of 404 and 36.7 nmoles MTX equivalence per 10(6) cells. The assay is proposed as an efficient approach to the detection and quantification of MTX-like compounds in Gambierdiscus spp.
SK&F 96365 曾被用于基于神经母细胞瘤（Neuro-2a 细胞）的检测方法，以确定两种属的双鞭甲藻（ Gambierdiscus spp.）中类似雪卡毒素（maitotoxin-like，MTX-like）化合物的产生情况。该检测方法在 2.5 小时内有效检测到 MTX 诱导的毒性效应，其半数细胞活力抑制浓度（IC（50））等效于 3.38 nM MTX。在研究的两种双鞭甲藻中，均发现了浓度为 404 和 36.7 毫摩尔 MTX 当量/10^6 个细胞的类似雪卡毒素化合物的产生。该检测方法被提议作为一种有效方法，用于检测和定量双鞭甲藻中的类似雪卡毒素化合物。
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