Department of Anatomy and Histology, Anderson Stuart Building F13, School of Medical Sciences, Bosch Institute, The University of Sydney, Sydney, NSW, 2006, Australia.
Histochem Cell Biol. 2010 May;133(5):549-55. doi: 10.1007/s00418-010-0693-y. Epub 2010 Mar 24.
Adhesion molecules play an important part in preparing uterine epithelial cells for receptivity to the implanting embryo, and their rearrangement is crucial in allowing successful implantation. CD43 is an adhesion molecule which has previously been suggested to take part in implantation in mice. Indirect immunofluorescence microscopy localising CD43 was performed on uterine tissue during early pregnancy, and tissue obtained from ovariectomised rats administered with ovarian hormones. Western blotting was performed during early pregnancy on isolated epithelial cells and ovariectomised rats for comparison of the amount of CD43. Immunofluorescence microscopy showed CD43 was situated basally in uterine luminal epithelial cells on day 1 of pregnancy and during oestrogen administration, corresponding to a 95-kDa band of CD43 seen in western blotting. At the time of implantation, and during progesterone or progesterone plus oestrogen combined treatment, CD43 is apical in uterine luminal epithelial cells, resulting in an 85-kDa form of CD43. We suggest that a de-glycosylated form of CD43 moves from basally to apically at the time of implantation, thus facilitating blastocyst attachment to uterine epithelial cells as well as their removal.
黏附分子在使子宫内膜上皮细胞为着床胚胎做好接受准备方面发挥着重要作用，其重排对于成功着床至关重要。CD43 是一种黏附分子，先前有研究表明其在小鼠的着床过程中发挥作用。本研究通过间接免疫荧光显微镜技术，对妊娠早期的子宫组织和接受卵巢激素处理的卵巢切除大鼠的组织进行 CD43 定位。在妊娠早期，通过 Western blot 对分离的上皮细胞和卵巢切除大鼠进行分析，比较 CD43 的含量。免疫荧光显微镜显示，妊娠第 1 天和雌激素处理时，CD43 位于子宫腔上皮细胞的基底侧，Western blot 显示相应的 CD43 为 95kDa 带。在着床时，以及孕激素或孕激素加雌激素联合处理时，CD43 位于子宫腔上皮细胞的顶端，导致 CD43 形成 85kDa 形式。我们推测，在着床时，CD43 的去糖基化形式从基底侧向顶端转移，从而促进囊胚附着于子宫上皮细胞并将其去除。
