School of Environmental Science and Engineering, Shanghai Jiao-Tong University, Shanghai 200240, China.
J Ind Microbiol Biotechnol. 2010 Jun;37(6):609-16. doi: 10.1007/s10295-010-0708-7. Epub 2010 Mar 24.
Providencia rettgeri strain YL was found to be efficient in heterotrophic nitrogen removal under aerobic conditions. Maximum removal of NH(4) (+)-N occurred under the conditions of pH 7 and supplemented with glucose as the carbon source. Inorganic ions such as Mg(2+), Mn(2+), and Zn(2+) largely influenced the growth and nitrogen removal efficiency. A quantitative detection of nitrogen gas by gas chromatography was conducted to evaluate the nitrogen removal by strain YL. From the nitrogen balance during heterotrophic growth with 180 mg/l of NH(4) (+)-N, 44.5% of NH(4) (+)-N was in the form of N(2) and 49.7% was found in biomass, with only a trace amount of either nitrite or nitrate. The utilization of nitrite and nitrate during the ammonium removal process demonstrated that the nitrogen removal pathway by strain YL was heterotrophic nitrification-aerobic denitrification. A further enzyme assay of nitrate reductase and nitrite reductase activity under the aerobic condition confirmed this nitrogen removal pathway.
产酸克雷伯氏菌 YL 菌株在好氧条件下对异养脱氮具有较高的去除效率。在 pH 值为 7 并添加葡萄糖作为碳源的条件下，NH(4) (+)-N 的去除率最高。无机离子如 Mg(2+)、Mn(2+)和 Zn(2+) 对生长和脱氮效率有很大影响。通过气相色谱法对氮气进行定量检测，以评估 YL 菌株的脱氮能力。从异养生长过程中的氮平衡来看，在 180mg/L 的 NH(4) (+)-N 条件下，44.5%的 NH(4) (+)-N 以 N(2)的形式存在，49.7%存在于生物量中，只有痕量的亚硝酸盐或硝酸盐。在铵去除过程中对亚硝酸盐和硝酸盐的利用表明，YL 菌株的脱氮途径是异养硝化-好氧反硝化。在好氧条件下进一步对硝酸还原酶和亚硝酸盐还原酶活性进行酶测定，证实了这一氮去除途径。
