Eye Research Institute Maastricht, Department of Ophthalmology, University Hospital Maastricht, Maastricht, The Netherlands.
Invest Ophthalmol Vis Sci. 2010 Aug;51(8):4143-50. doi: 10.1167/iovs.09-5057. Epub 2010 Mar 24.
Interleukin (IL)-6, a multifunctional cytokine with regulatory functions in wound healing, and several chemokines have been implicated in the pathogenesis of proliferative vitreoretinopathy (PVR) after rhegmatogenous retinal detachment (RRD). The exact role of these chemokines, their correlation with IL-6 after primary RRD, and their association with the future development of PVR are not yet known.
A multiplex immunoassay was used to determine levels of 15 different chemokines and IL-6 in subretinal fluid samples obtained during scleral buckling surgery for primary RRD. Samples from patients with preoperative uveitis, preoperative trauma, or preoperative vitreous hemorrhage were excluded. Patients who developed a redetachment due to postsurgical PVR within 2.5 months (n = 21) were compared with control subjects who had an uncomplicated retinal detachment during the overall follow-up period (n = 54). Control subjects were matched for sex, age, and storage time.
Levels of IL-6 (P = 0.001), MIF (P = 0.016), CCL2 (P = 0.041), CCL11 (P = 0.012), CCL17 (P = 0.003), CCL18 (P = 0.007), CCL19 (P < 0.001), CCL22 (P < 0.001), CXCL8 (P = 0.027), CXCL9 (P = 0.007), and CXCL10 (P = 0.002) were significantly higher in patients who developed postoperative PVR after primary RRD than in patients with uncomplicated retinal detachment. A significant positive correlation was observed between IL-6 and both CCL22 (r = 0.538; P < 0.0001) and CXCL8 (r = 0.645; P < 0.0001).
Various chemokines and IL-6 are upregulated in patients in whom fibrotic membranes develop after primary RRD repair and may therefore be involved in the future development of postoperative PVR.
白细胞介素（IL）-6 是一种具有多种功能的细胞因子，在伤口愈合过程中具有调节作用，几种趋化因子也与孔源性视网膜脱离（RRD）后增生性玻璃体视网膜病变（PVR）的发病机制有关。这些趋化因子的确切作用、它们与原发性 RRD 后 IL-6 的相关性以及它们与 PVR 未来发展的相关性尚不清楚。
使用多重免疫测定法测定巩膜扣带术治疗原发性 RRD 时获得的视网膜下液样本中 15 种不同趋化因子和 IL-6 的水平。排除术前存在虹膜炎、创伤或玻璃体积血的患者。在 2.5 个月内因手术后 PVR 再次发生脱离的患者（n = 21）与整个随访期间视网膜脱离无并发症的对照组患者（n = 54）进行比较。对照组患者按性别、年龄和储存时间进行匹配。
IL-6（P = 0.001）、MIF（P = 0.016）、CCL2（P = 0.041）、CCL11（P = 0.012）、CCL17（P = 0.003）、CCL18（P = 0.007）、CCL19（P < 0.001）、CCL22（P < 0.001）、CXCL8（P = 0.027）、CXCL9（P = 0.007）和 CXCL10（P = 0.002）水平在原发性 RRD 后发生术后 PVR 的患者中明显高于视网膜脱离无并发症的患者。IL-6 与 CCL22（r = 0.538；P < 0.0001）和 CXCL8（r = 0.645；P < 0.0001）呈显著正相关。
各种趋化因子和 IL-6 在原发性 RRD 修复后形成纤维膜的患者中上调，因此可能参与术后 PVR 的发生发展。
